Self-assembling complex for targeting chemical agents to cells
The present invention relates to a complex that can be injected into the body to hone in on target cells to deliver molecules. In one embodiment, the invention provides a drug delivery system that includes components that self-assemble into one targeted conjugate. In another embodiment, the invention includes a targeted carrier protein and a nucleic acid sequence non-covalently linked to one or more drugs.
1. A drug delivery molecule, comprising:
a polypeptide sequence adapted to target and/or penetrate a type of cell;
a nucleic acid sequence bound to a polypeptide sequence via electrostatic interactions; and
a chemical agent intercalated with a nucleic acid sequence.
2. The drug delivery molecule of claim 1 , wherein the polypeptide sequence comprises a targeting ligand.
3. The drug delivery molecule of claim 1 , wherein the polypeptide sequence comprises an endosomolytic domain.
4. The drug delivery molecule of claim 1 , wherein the polypeptide sequence comprises a positively charged domain.
5. The drug delivery molecule of claim 1 , wherein the polypeptide sequence comprises a polylysine motif.
6. The drug delivery molecule of claim 1 , wherein the chemical agent is doxorubicin.
7. The drug delivery molecule of claim 1 , wherein the type of cell is HER2+ breast cancer cell.
8. A self-assembling complex, comprising:
a recombinant fusion protein; and
a double stranded oligonucleotide, bound to the recombinant fusion protein by electrostatic interactions, wherein the double-stranded oligonucleotide is intercalated with a chemical agent.
9. The self-assembling complex of claim 8 , wherein the recombinant fusion protein comprises a Her segment.
10. The self-assembling complex of claim 8 , wherein the recombinant fusion protein comprises a penton base segment.
11. The self-assembling complex of claim 8 , wherein the recombinant fusion protein comprises a decalysine segment.
12. A composition comprising:
a drug delivery molecule comprising
a polypeptide sequence adapted to target and/or penetrate a type of cell,
a nucleic acid sequence bound to the polypeptide sequence via electrostatic interactions, and
a chemical agent intercalated with the nucleic acid sequence; and
a carrier.
13. The composition of claim 12 , wherein the polypeptide sequence comprises an endosomolytic domain.