O-acetylhomoserine sulfhydrylase or mutant protein thereof, and method for converting to methionine using the same
The present invention relates to a novel protein having O-acetylhomoserine sulfhydrylase activity, a mutant protein thereof, a polynucleotide encoding the same, a recombinant vector comprising the polynucleotide, a microorganism transformed with the recombinant vector, and a method for producing methionine or acetic acid using the protein. The production method of the present invention has the advantage of producing L-methionine and acetic acid cost-effectively through having higher conversion rate and reduced reaction time compared to the existing methods, and it can minimize the amount of enzyme homogenate added when using the mutant protein, thereby easily producing L-methionine and acetic acid at high yield.
1. A mutant protein of a polypeptide of SEQ ID No. 13 having O-acetylhomoserine sulfhydrylase activity, wherein said mutant protein has all of SEQ ID No. 13 except for one or more substitution selected from the group consisting of substitution of the isoleucine at position 3 with an amino acid other than isoleucine, substitution of the phenylalanine at position 65 with tyrosine, and substitution of the valine at position 104 with an amino acid other than valine.
2. The mutant protein according to claim 1 , wherein the mutation is made by substitution of the 3 rd amino acid from the N-terminal with asparagine, and substitution of the 104 th amino acid with alanine.
3. The mutant protein according to claim 1 , having the amino acid sequence selected from the group consisting of SEQ ID Nos. 15 to 18.
4. A polynucleotide encoding the mutant protein of claim 1 .
5. The polynucleotide according to claim 4 having the nucleotide sequence selected from the group consisting of SEQ ID Nos. 19 to 22.
6. A recombinant vector comprising the polynucleotide of claim 4 .
7. A microorganism transformed with the recombinant vector of claim 6 for producing O-acetylhomoserine sulfhydrylase.
8. The microorganism according to claim 7 , which is Escherichia coli ( E. coli ).
9. A method for producing methionine or acetic acid, comprising adding a protein having O-acetylhomoserine sulfhydrylase activity and comprising the amino acid sequence of SEQ ID No. 13 or a microorganism producing the same to a mixture of O-acetylhomoserine and methyl mercaptan to thereby react the resulting mixture.
10. A recombinant vector comprising the polynucleotide of claim 5 .
11. A method for producing methionine or acetic acid, comprising adding a protein of claim 1 or a microorganism producing the same to a mixture of O-acetylhomoserine and methyl mercaptan to thereby react the resulting mixture.
12. A method for producing methionine or acetic acid, comprising adding a protein of claim 3 or a microorganism producing the same to a mixture of O-acetylhomoserine and methyl mercaptan to thereby react the resulting mixture.