Formulations of viable microorganisms and their methods of production and use
View Patent ↗The present invention relates to formulations of viable microorganisms in a water insoluble, water-absorbent substance and methods of producing formulations of viable microorganisms. Also disclosed is a method of treating plants and/or plant seeds with the formulations of the present application.
1. A powder formulation of viable microorganisms comprising:
(a) microencapsulated microorganisms comprising:
(i) microorganisms in an amount of at least 5×10 8 colony forming units per gram of the formulation, and
(ii) a water-soluble encapsulating material microencapsulating the microorganisms; and
(b) a water insoluble, water-absorbent substance that is a dry, free-flowing powder mixed with the microencapsulated microorganisms, the water insoluble, water-absorbent substance present in an amount sufficient to maintain the formulation as a dry, free-flowing powder.
2. The formulation according to claim 1 , wherein the water insoluble, water-absorbent substance is selected from the group consisting of zeolite, porous beads or powders, silica, ground agricultural products, porous wood products, cellulose, cyclodextrins, and combinations thereof.
3. The formulation according to claim 2 , wherein the water insoluble, water-absorbent substance is cellulose powder.
4. The formulation according to claim 1 , wherein the microorganisms are selected from the group consisting of fungi, bacteria, and combinations thereof.
5. The formulation according to claim 4 , wherein the microorganisms are fungi.
6. The formulation according to claim 5 , wherein one of the fungi comprises a species from the genus Trichoderma.
7. The formulation according to claim 4 , wherein the microorganisms are bacteria.
8. The formulation according to claim 7 , wherein one of the bacteria comprises a species from the genus Bradyrhizobium.
9. The formulation according to claim 1 , wherein the encapsulating material is present, by total weight of the formulation, in an amount of at least 1% (w/w).
10. The formulation according to claim 1 , wherein the encapsulating material is selected from the group consisting of a chitosan, a starch, a glucan, a dextrin, a cellulose which is water soluble, a vegetable or microbial gum, and combinations thereof.
11. The formulation according to claim 1 , wherein the water insoluble, water-absorbent substance is present, by total weight of the formulation, in an amount of at least 90% (w/w).
12. A powder formulation of viable bacteria comprising:
(a) microencapsulated bacteria, comprising:
(i) bacteria in an amount of at least 5×10 8 colony forming units per gram of the formulation, the bacteria comprising at least one species of the genus Bradyrhizobium , and
(ii) a water-soluble encapsulating material microencapsulating the bacteria, the water-soluble encapsulating material selected from the group consisting of a chitosan, a starch, a glucan, a dextrin, a cellulose which is water soluble, a vegetable or microbial gum, and combinations thereof; and
(b) cellulose powder, mixed with the microencapsulated bacteria, the cellulose powder present, by total weight of the formulation, in an amount of at least 90% (w/w) to maintain the formulation as a dry, free-flowing powder.
13. The formulation of claim 12 , wherein the water-soluble encapsulating material is a dextrin.