Composition for anti-aging or antioxidant composition containing plant stem cell line derived from cambium or
The present invention relates to an anti-aging or antioxidant composition which contains, as an active ingredient, a plant stem cell line derived from the cambium of Panax ginseng , including wild ginseng and ginseng, or an extract thereof, a lysate thereof and a culture thereof. The composition has minimized side effects compared to existing anti-aging agents and antioxidants, and thus is safe for the skin. Also, the composition of the present invention shows an antioxidant effect of inhibiting reactive oxygen species caused by exposure to UV radiation that is the major cause of skin aging, and it can effectively reduce or inhibit aging-related factors. Thus, the composition is useful for the prevention and inhibition of aging.
1. A method of inhibiting aging or oxidation of the skin of a subject, comprising:
administering, to the skin of the subject, a composition comprising one or more of an extract, a culture or a lysate of a homogeneous preparation of innately undifferentiated cells of a cell line derived from a cambium of Panax ginseng , thereby inhibiting the aging or oxidation of the skin of the subject.
2. The method according to claim 1 , wherein the cell line is additionally characterized in that:
(a) the cells are present as single cells during suspension culture;
(b) the cells have low sensitivity to shear stress in a bioreactor compared to cell lines derived from tissues other than the cambium of Panax ginseng ; and
(c) the cells have a higher growth rate than those of cell lines derived from tissues other than the cambium of Panax ginseng and are cultured stably.
3. The method according to claim 1 , wherein the cell line is obtained using an isolation method comprising the following steps of:
(a) obtaining a storage root tissue containing the cambium of Panax ginseng;
(b) applying osmotic stress to the obtained cambium-containing storage root tissue, and then culturing the cambium-containing storage root tissue in a IAA (Indole-3-acetic acid)- or IBA (Indole-3-butyric acid)-containing medium, thereby inducing a cambium-derived cell line; and
(c) collecting the induced cambium-derived cell line.
4. The method according to claim 3 , wherein the culture is obtained by additionally culturing the cell line in a medium which contains 3-5 wt. % of raw sugar or sugar; or at least one substance selected from the group consisting of methyl jasmonate, fungal extract, bacterial extract, yeast extract, chitosan, glucomannan, glucan, phenylalanine, benzoic acid, salicylic acid, arachidonic acid, STS (staurosporine), mevalonalonate N-benzolyglycine, ABA (aminobenzoic acid), SNP (sodium nitroprusside), IPP (isopentenyl pyrophosphate), BHT (butylated hydroxytoluene), CCC (chlorocholine chloride), ethephon, hippuric acid, ammonium ceric nitrate, AgNO 3 , vanadyl sulfate, p-aminobenzoic acid, brassinosteroids, sodium alginate, and sodium acetate.
5. The method according to claim 1 , wherein the Panax ginseng is wild ginseng or ginseng.
6. The method according to claim 1 , wherein the extract is obtained using a solvent selected from the group consisting of distilled water, alcohol, acetone, DMSO (dimethyl sulfoxide), and mixtures thereof.
7. The method according to claim 1 , wherein the extract is obtained by fractionating the cells sequentially with distilled water, methanol and acetone.
8. The method according to claim 1 , wherein the cell line is obtained using an isolation method comprising the following steps of:
(a) obtaining a storage root tissue containing the cambium of Panax ginseng;
(b) applying osmotic stress to the obtained cambium-containing storage root tissue, and then culturing the cambium-containing storage root tissue in a IAA (Indole-3-acetic acid)-containing medium, thereby inducing a cambium-derived cell line; and
(c) collecting the induced cambium-derived cell line.
9. The method according to claim 8 , wherein the culture is obtained by additionally culturing the cell line in a medium which contains 3-5 wt. % of raw sugar or sugar; or methyl jasmonate.