IP Library › Granted Patent US 9,095,554
Granted Patent B2
US 9,095,554 · App. 14/216,744 · Granted Aug 4, 2015

Breeding, production, processing and use of specialty cannabis

Inventors: Mark Anthony Lewis (Westlake Village, CA); Michael D Backes (Westlake Village, CA); Matthew Giese (Westlake Village, CA)
Assignee: Biotech Institute LLC
A61K36/185A01H1/04A01H4/00A01H5/02A01H5/12
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Quick Facts
Patent No.
US 9,095,554
App. No.
14/216,744
Granted
Aug 4, 2015
Kind
B2
Abstract

The invention provides compositions and methods for the breeding, production, processing and use of specialty cannabis.

Claims (30)

1. A hybrid cannabis plant, or an asexual clone of said hybrid cannabis plant, or a plant part, tissue, or cell thereof, which produces a female inflorescence, said inflorescence comprising:

a) a B T /B D genotype;

b) a terpene profile in which myrcene is not the dominant terpene;

c) a terpene oil content greater than about 1.0% by weight; and

d) a CBD content greater than 3%;

wherein the terpene profile is defined as terpinolene, alpha phelladrene, beta ocimene, careen, limonene, gamma terpinene, alpha pinene, alpha terpinene, beta pinene, fenchol, camphene, alpha terpineol, alpha humulene, beta caryophyllene, linalool, cary oxide, and myrcene, and wherein the terpene oil content is determined by the additive content of the terpenes in the terpene profile; and wherein the terpene contents and CBD content are measured by gas chromatography-flame ionization detection (GC-FID) and calculated based on dry weight of the inflorescence; wherein a representative sample of seed producing said plants has been deposited under NCIMB Nos. 42246, 42247, 42248, 42249, 42250, and 42254.

2. The hybrid cannabis plant, or an asexual clone of said hybrid cannabis plant, or a plant part, tissue, or cell thereof of claim 1 wherein the plant, plant part, tissue, or cell comprises a terpene oil content greater than about 1.5% by weight.

3. The hybrid cannabis plant, or an asexual clone of said hybrid cannabis plant, or a plant part, tissue, or cell thereof of claim 1 , wherein the hybrid cannabis plant, or an asexual clone of said hybrid cannabis plant, or a plant part, tissue, or cell thereof comprises a tetrahydrocannabinol (THC) content that is at least 3.0% by weight as measured by GC-FID and calculated based on dry weight of the inflorescence.

4. The hybrid cannabis plant, or an asexual clone of said hybrid cannabis plant, or a plant part, tissue, or cell thereof of claim 1 , wherein the hybrid cannabis plant, or an asexual clone of said hybrid cannabis plant, or a plant part, tissue, or cell thereof comprises a tetrahydrocannabinol (THC) content that is at least 6.0% by weight as measured by GC-FID and calculated based on dry weight of the inflorescence.

5. The hybrid cannabis plant, or an asexual clone of said hybrid cannabis plant, or a plant part, tissue, or cell thereof of claim 1 , wherein the hybrid cannabis plant, or an asexual clone of said hybrid cannabis plant, or a plant part, tissue, or cell thereof comprises a cannabidiol CBD content that is at least 6.0% by weight as measured by GC-FID and calculated based on dry weight of the inflorescence.

6. The hybrid cannabis plant, or an asexual clone of said hybrid cannabis plant, or a plant part, tissue, or cell thereof of claim 1 , wherein limonene is the most abundant terpene in said terpene profile.

7. The hybrid cannabis plant, or an asexual clone of said hybrid cannabis plant, or a plant part, tissue, or cell thereof of claim 1 , wherein terpinolene is the most abundant terpene in said terpene profile.

8. The hybrid cannabis plant, or an asexual clone of said hybrid cannabis plant, or a plant part, tissue, or cell thereof of claim 1 , wherein alpha pinene is the most abundant terpene in said terpene profile.

9. The hybrid cannabis plant, or an asexual clone of said hybrid cannabis plant, or a plant part, tissue, or cell thereof of claim 1 , wherein beta caryophyllene is the most abundant terpene in said terpene profile.

10. A method of breeding chemotype II cannabis plants with a non-myrcene dominant terpene profile, said method comprising:

(i) making a cross between a first hybrid cannabis plant, or an asexual clone of said hybrid cannabis plant of claim 1 and a second cannabis plant to produce an F1 seed:

(ii) harvesting the resulting seed;

(iii) growing said seed; and

(iv) selecting a cannabis plant with a desired phenotype;

wherein the resulting selected cannabis plant is a chemotype II cannabis plant with a non-myrcene dominant terpene profile.

11. A method of producing a chemotype II cannabis plant with a non-myrcene dominant terpene profile, said method comprising:

(i) obtaining a cannabis seed, cutting, or plant cell, from a hybrid cannabis plant, or an asexual clone of said hybrid cannabis plant of claim 1 ;

(ii) placing said cannabis seed, cutting, or plant cell in an environment conducive to growth; and

(iii) allowing said cannabis seed, cutting, or plant cell to produce a cannabis plant;

wherein said produced cannabis plant is a chemotype II cannabis plant with a non-myrcene dominant terpene profile.

12. A cannabis extract from the hybrid cannabis plant, or an asexual clone of said hybrid cannabis plant, or a plant part, tissue, or cell thereof of claim 1 .

13. The cannabis extract of claim 12 , wherein said extract is selected from the group consisting of kief, hashish, bubble hash, solvent reduced oils, sludges, e-juice, and tinctures.

14. An edible product comprising cannabis tissue from the hybrid cannabis plant, or an asexual clone of said hybrid cannabis plant, or a plant part, tissue, or cell thereof of claim 1 .

15. An edible product comprising the cannabis extract of claim 12 .

16. The hybrid cannabis plant, or an asexual clone of said hybrid cannabis plant, or a plant part, tissue, or cell thereof of claim 1 , wherein said plant part is selected from the group consisting of: trichomes, sun leaves, or inflorescences.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 17, 2014
From: LEWIS, MARK A; GIESE, MATTHEW; BACKES, MICHAEL DANE
To: BIOTECH INSTITUTE, LLC
Reel/Frame 032457/0439 →
Continuity (3)
Provisional Application 61897074 · Oct 29, 2013
Provisional Application 61801528 · Mar 15, 2013
Related Publication 20140287068A1 · Sep 25, 2014