Photocleavable linker
There are provided, inter alia, photolabile compounds and methods useful for the formation of dimers of biological molecules and subsequent dissociation of the dimers.
1. A compound having the formula:
wherein,
L 1 is a photocleavable linker, and z 1 and z 2 are each independently an integer in the range 0 to 6.
2. The compound of claim 1 , wherein
L 1 is substituted or unsubstituted alkylene, substituted or unsubstituted heteroalkylene, substituted or unsubstituted cycloalkylene, substituted or unsubstituted heterocycloalkylene, substituted or unsubstituted arylene or substituted or unsubstituted heteroarylene.
3. The compound of claim 1 , wherein L 1 comprises a nitrophenylene.
4. The compound of claim 1 , wherein L 1 has the formula:
-L 2 -L 3 -L 4 -L 5 -L 6 -
wherein
L 2 and L 6 are —C(O)O;
L 3 and L 5 are independently a bond or substituted or unsubstituted alkylene;
L 4 is R 1 -substituted arylene; and
R 1 is an electron withdrawing group.
5. The compound of claim 4 , wherein
R 1 is halogen, —NO 2 , N + (R 2 ) 3 , —SR 2 , —OR 2 , —N(R 2 ) 2 , —CF 3 , —CCl 3 , —CN, —SO 3 R 2 , —COOR 2 , —CHO or —COR 2 ; and
R 2 is hydrogen or an unsubstituted C 1 to C 4 alkyl.
6. The compound of claim 4 , wherein L 1 is:
7. The compound of claim 4 , wherein L 3 and L 5 are independently substituted or unsubstituted alkylene.
8. The compound of claim 4 , wherein L 3 and L 5 are independently substituted or unsubstituted C 1 to C 4 alkylene.
9. The compound of claim 4 , wherein L 3 and L 5 are independently substituted or unsubstituted C 1 to C 3 alkylene.
10. The compound of claim 4 , wherein L 3 and L 5 are substituted with an unsubstituted C 1 to C 4 alkylene.
11. The compound of claim 4 , wherein L 3 and L 5 are substituted with a methyl.
12. The compound of claim 4 , wherein L 3 and L 5 are methylene substituted with an unsubstituted C 1 to C 4 alkylene.
13. The compound of claim 4 , wherein L 1 is:
14. The compound of claim 4 , wherein R 1 is NO 2 .
15. The compound of claim 1 having the formula:
16. The compound of claim 1 having the formula:
17. The compound of claim 1 having the formula:
18. A method of forming an FK506 binding protein (FKBP) dimer, the method comprising:
(i) contacting a first FKBP and a second FKBP with the compound of claim 1 ; and
(ii) allowing said compound to bind to said first FKBP and said second FKBP thereby forming an FKBP dimer comprising a link between said first FKBP and said second FKBP.
19. The method of claim 18 , further comprising:
(iii) exposing said FKBP dimer to a photon thereby cleaving said link.