Method and system for concentrating particles from a solution
Methods and systems are provided for concentrating particles (e.g., bacteria, viruses, cells, and nucleic acids) suspended in a liquid. Electric-field-induced forces urge the particles towards a first electrode immersed in the liquid. When the particles are in close proximity to (e.g., in contact with) the first electrode, the electrode is withdrawn from the liquid and capillary forces formed between the withdrawing electrode and the surface of the liquid immobilize the particles on the electrode. Upon withdrawal of the electrode from the liquid, the portion of the electrode previously immersed in the liquid has particles immobilized on its surface.
1. A method for concentrating a nucleic acid, comprising:
(a) immersing a first electrode having a high aspect ratio in a liquid comprising a nucleic acid, wherein the first electrode comprises a shaft having a shaft latitudinal dimension and a distal end having a distal latitudinal dimension, wherein the distal latitudinal dimension is from one nanometer to one millimeter;
(b) urging the nucleic acid toward the first electrode by generating an electric-field-induced force using the first electrode; and
(c) immobilizing the nucleic acid on a surface of the first electrode with a capillary force formed between the first electrode and the liquid by withdrawing the first electrode from the liquid;
wherein the electric-field-induced force is created by an electric field that is maintained through the step of immobilizing the nucleic acid on a surface of the first electrode with a capillary force formed between the first electrode and the liquid by withdrawing the first electrode from the liquid.
2. The method of claim 1 further comprising analyzing the nucleic acid immobilized on the surface of the first electrode.
3. The method of claim 2 , wherein analyzing the nucleic acid comprises a method selected from the group consisting of electrical, mechanical, optical, surface-imaging techniques, and combinations thereof.
4. The method of claim 3 , wherein analyzing the nucleic acid by an optical method comprises attaching a luminescent compound to the nucleic acid to provide a luminescent particle and detecting luminescence from the luminescent particle.
5. The method of claim 3 , wherein analyzing the nucleic acid by an electrical method comprises a technique for measuring a characteristic selected from the group consisting of capacitance, resistance, conductance, impedance, and combinations thereof.
6. The method of claim 1 , wherein the first electrode comprises a material selected from the group consisting of a metal, a doped semiconductor, and a conductive polymer.
7. The method of claim 1 , wherein the latitudinal dimension of the first electrode is larger than a longitudinal dimension of the nucleic acid.
8. The method of claim 1 , wherein the first electrode is at least partially coated with a surface coating.
9. The method of claim 8 , wherein the surface coating is selected from the group consisting of a monolayer and a polymer layer.
10. The method of claim 8 , wherein the surface coating enhances the immobilization of the nucleic acid on the first electrode.
11. The method of claim 10 , wherein the surface coating comprises a first binding partner and the nucleic acid comprises a second binding partner capable of binding to the first binding partner.
12. The method of claim 11 , wherein the first binding partner is a first nucleic acid.
13. The method of claim 11 , wherein the first binding partner is a protein.
14. The method of claim 1 , wherein the electric-field-induced force is selected from the group consisting of electrophoresis, electroosmosis, dielectrophoresis, and combinations thereof.
15. The method of claim 1 , wherein the electric-field-induced force is generated between the first electrode and a second electrode in contact with the liquid.
16. The method of claim 1 , wherein the liquid is selected from the group consisting of a solution and a suspension.
17. The method of claim 1 , wherein the liquid is a biological fluid selected from the group consisting of blood, sputum, mucous, and saliva.
18. The method of claim 1 , wherein the nucleic acid is DNA or RNA.