Device and methods of detection of airborne agents
Provided are methods, devices and systems that utilize free-surface fluidics and SERS for analyte detection with high sensitivity and specificity. The molecules can be airborne agents, including but not limited to explosives, narcotics, hazardous chemicals, or other chemical species. The free-surface fluidic architecture is created using an open microchannel, and exhibits a large surface to volume ratio. The free-surface fluidic interface can filter interferent molecules, while concentrating airborne analyte molecules. The microchannel flow enables controlled aggregation of SERS-active probe particles in the flow, thereby enhancing the detector's sensitivity.
1. A system comprising:
a microfluidic device comprising:
a non-flowing fluid;
one or more free-surface interface regions that are open on at least one side to atmospheric air, wherein the non-flowing fluid is located on the one or more free-surface interface regions, and wherein at least a portion of the fluid is exposed to atmospheric air so that one or more analytes from a sample can come into contact with the surface of the fluid and be absorbed into the fluid; and
means for detecting the presence of the one or more analytes absorbed in the fluid.
2. The system of claim 1 , wherein the means detects the presence of one or more analytes absorbed in the fluid by a technique selected from the group consisting of Raman spectroscopy or surface enhanced Raman scattering (“SERS”) measurements of a probe that is contained in the fluid; electrochemical analysis techniques; fluorescent chemical marker techniques; fluorescence quenching; redox-labeled nucleic acid binding techniques; X-Ray absorption techniques; IR, visible, and UV electromagnetic radiation absorption techniques; mass spectroscopy techniques; liquid chromatography techniques; flame ionization analysis techniques; DNA melting point techniques; and titration analysis techniques.
3. The system of claim 1 , wherein the fluid comprises one or more nanoparticles.
4. The system of claim 3 , wherein the one or more nanoparticles are functionalized.
5. The system of claim 4 , wherein the one or more nanoparticles are functionalized by imprinting one or more functional groups.
6. The system of claim 5 , wherein the one or more functional groups are selected from the group consisting of antibodies, proteins, nucleic acids, carbohydrates, alkanethiols, cyclohexyl mercaptans, glucosamines, boronic acids, mercapto carboxylic acids, and mixtures thereof.
7. The system of claim 5 , wherein the one or more functional groups comprise components for specific interactions with a specific analyte.
8. The system of claim 7 , wherein the one or more functional groups comprise surface bound targeting ligands for interacting with specific analytes so as to form an analyte/targeting ligand pair.
9. The system of claim 8 , wherein the interaction between the analyte/targeting ligand pair allows for detection of the analyte by SERS.
10. The system of claim 8 , wherein the analyte/targeting ligand pair is selected from the group consisting of antibodies/antigens, receptors/ligands, proteins/nucleic acids, nucleic acids/nucleic acids, enzymes/substrates, enzymes/inhibitors, carbohydrates/lectins, carbohydrates/proteins, proteins/proteins, and protein/small molecules.
11. The system of claim 4 , wherein one or more nanoparticles are functionalized by forming a self-assembled monolayer (“SAM”) on one or more surfaces.
12. The system of claim 11 , wherein the SAM is selected from the group consisting of n-alkanethiols, 4-aminothiophenol, L-cysteine, 3-mercaptopropionic acid, 11-mercaptoundecanoic acid, 1-hexanethiol, 1-octanethiol, 1-DT, 1-hexadecanethiol, poly-DL-lysine, 3-mercapto-1-propanesufonic acid, benzenethiol, cyclohexylmercaptan, and mixtures thereof.
13. The system of claim 4 , wherein one or more nanoparticles are functionalized by coating one more surfaces with one or more compounds to inhibit the accumulation of biological material.
14. The system of claim 13 , wherein the one or more compounds are selected from the group consisting of polyethyleneglycol (“PEG”), silane terminated monomethoxyPEG, oligoethyleneglycol terminated alkanethiols, and mixtures thereof.