Method for determining glucagon-like peptide-1, and kit for use in said method
Provided is a method of measuring the presence and/or the amount of glucagon-like peptide-1 (GLP-1) in a sample, which method is characterized by comprising the step of treating the sample in advance with an acidic solution, and a kit of measuring the presence and/or an amount of GLP-1 in a sample, the kit containing (a) the acidic solution, (b) an antibody specific to GLP-1, and (c) an instruction manual.
1. A method of measuring the presence and/or an amount of glucagon-like peptide-1 (GLP-1) in a sample isolated from a subject, comprising treating said sample isolated from a subject with an acidic solution, neutralizing the acidic solution and then measuring the presence and/or an amount of GLP-1 in the sample, wherein the acidic solution has a pH of 2.5 or less.
2. The method according to claim 1 , wherein said acidic solution comprises at least one of glycine, hydrochloric acid, sulfuric acid, and phosphoric acid.
3. The method according to claim 1 , wherein said sample is blood.
4. The method according to claim 1 , wherein said method of measuring GLP-1 comprises an enzyme immunoassay or a competitive radioimmunoassay.
5. A kit of measuring the presence and/or an amount of GLP-1 in a sample, comprising:
(a) an acidic solution having a pH of 2.5 or less,
(b) an antibody specific to GLP-1,
(c) an instruction manual, and
(d) an alkali solution for neutralization of the acidic solution.
6. The method according to claim 2 , wherein said sample is blood.
7. The method according to claim 2 , wherein said method of measuring GLP-1 comprises an enzyme immunoassay or a competitive radioimmunoassay.
8. The method according to claim 3 , wherein said method of measuring GLP-1 comprises an enzyme immunoassay or a competitive radioimmunoassay.
9. The method according to claim 6 , wherein said method of measuring GLP-1 comprises an enzyme immunoassay or a competitive radioimmunoassay.