Methods and compositions for selective regulation of protein expression
The invention provides methods and compositions for selectively suppressing the expression of a recombinant protein in a male reproductive tissue of a transgenic plant. The invention also provides methods and compositions for inducing male sterility in a transgenic plant. Plants, plant cells, plant parts, seeds, and commodity products including such compositions are aspects of the invention.
1. A recombinant DNA construct comprising a protein-coding sequence operably linked to a DNA sequence comprising a male tissue-specific siRNA (mts-siRNA) element, wherein said mts-siRNA element is heterologous with respect to said protein-coding sequence and comprises a DNA sequence selected from the group consisting of SEQ ID NO: 87 to 91.
2. The recombinant DNA construct of claim 1 , wherein the expression of said protein-coding sequence in a transgenic plant confers at least vegetative herbicide tolerance to said plant.
3. The recombinant DNA construct of claim 2 , wherein said protein-coding sequence encodes a glyphosate-tolerant EPSPS.
4. A method of making a recombinant DNA construct comprising
a) identifying an mts-siRNA element comprising at least one mts-siRNA sequence, and
b) operably linking said mts-siRNA element to a protein-coding sequence, wherein said mts-siRNA element is heterologous with respect to said protein-coding sequence and comprises a DNA sequence selected from the group consisting of SEQ ID NO: 87 to 91.
5. The method of claim 4 , wherein said mts-siRNA element is tassel-specific.
6. A transgenic plant having in its genome the recombinant DNA construct of claim 1 .
7. A seed, progeny, or plant part of the transgenic plant of claim 6 .
8. The transgenic plant of claim 6 , wherein said transgenic plant is a monocotyledonous plant.
9. The transgenic plant of claim 6 , wherein said transgenic plant is a maize plant.
10. A method of selectively suppressing the expression of a recombinant protein in a male reproductive tissue of a transgenic plant comprising expressing in said transgenic plant a recombinant DNA construct of claim 1 .
11. The method of claim 10 , wherein said male reproductive tissue is a tassel of a maize plant.
12. The method of claim 10 , wherein said mts-siRNA element comprises at least three mts-siRNA sequences.
13. The method of claim 10 , wherein the expression of said recombinant protein in a transgenic plant confers at least vegetative herbicide tolerance to said plant.
14. The method of claim 13 , wherein said recombinant protein is a glyphosate-tolerant EPSPS.
15. The transgenic plant of claim 6 , wherein said mts-siRNA element is recognized by at least one mts-siRNA produced from a dsRNA formed between transcripts of opposite polarity.
16. The transgenic plant of claim 6 , wherein said mts-siRNA element is recognized by at least one mts-siRNA, the production of which requires RNA-dependent RNA polymerase activity.