Lipoprotein lipase assay

1. A method for detecting activity of lipoprotein lipase in a biological sample of a subject comprising:

administering a dosage of an anti-coagulant to the subject;

gathering the biological sample;

adding the biological sample to a well of a well plate;

adding a first buffer to the well of the well plate, wherein the first buffer comprises 0.15 M NaCl, 20 mM Tris-HCl and 1.5% fatty acid-free BSA;

adding a second buffer to the well of the well plate, wherein the second buffer comprises 0.0125% Zwittergent;

adding a fluorescent substrate to the well of the well plate, wherein the substrate is 0.62 μM 4,4-difluoro-5-methyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (BODIPY) dye and a quencher in a TG backbone, and wherein the biological sample, first buffer, second buffer and substrate form a mixture;

incubating the well plate;

measuring a fluorescence signal of the mixture within the well of the well plate to obtain a measured fluorescence signal; and

correlating the measured fluorescence signal to a level of LPL activity.

2. The method of claim 1 , wherein the anti-coagulant is heparin.

3. The method of claim 1 , wherein the anti-coagulant is administered intravenously, subcutaneously or a combination of intravenously and subcutaneously.

4. The method of claim 1 , wherein the well plate is incubated for a period between about 2 minutes to about 2 hours.

5. The method of claim 4 , wherein the well plate is incubated for about 10 minutes.

6. The method of claim 1 , wherein the biological sample is a blood sample.

7. The method of claim 1 , wherein the biological sample is a plasma portion of a blood sample.

8. The method of claim 1 , wherein the measured fluorescence signal-to-noise ratio (SNR) is about 30.

9. The method of claim 8 , wherein the SNR is about 30 after the well plate is incubated for about 10 minutes.