Multitype HPV peptide compositions and methods for treatment or prevention of human papillomavirus infection
Embodiments of the invention are directed to methods and compositions of multitype HPV polypeptides.
1. An isolated polypeptide composition comprising a fusion of residues from at least three homologous human papillomavirus L2 immunogenic peptides from at least three isolates of human papilloma virus wherein a first immunogenic peptide is coupled to a second homologous immunogenic peptide from a second isolate of human papilloma virus and the second immunogenic homologous peptide is coupled to a third homologous immunogenic peptide from a third isolate of human papilloma virus and wherein the immunogenic peptides are selected from one or more of:
L2 residues 11-200 from HPV6, HPV16 and HPV18; or
L2 residues 11-88 from HPV1, HPV5, HPV6, HPV16, HPV18; or
L2 residues 17-36 from HPV1, HPV2, HPV5, HPV6, HPV8, HPV11,
HPV16, HPV18, HPV31, HPV33, HPV35, HPV39, HPV45,
HPV51, HPV52, HPV56, HPV58, HPV59, HPV63, HPV68,
HPV73 and HPV82; or
L2 residues 11-88 from HPV6, HPV16, HPV18, HPV31, HPV39, HPV51, HPV56 and HPV73.
2. The polypeptide composition of claim 1 , wherein the immunogenic peptides are configured in a linear arrangement.
3. The polypeptide composition of claim 1 , wherein the immunogenic peptides are coupled through a linker moiety.
4. The polypeptide composition of claim 1 , wherein the polypeptide composition is a fusion protein.
5. The polypeptide composition of claim 4 , wherein the fusion protein contains a peptide linker coupling the immunogenic peptides.
6. The polypeptide composition of claim 1 , wherein the papillomavirus is a member of a papillomavirus genus selected from α, β, γ, δ, ε, ζ, η, θ, ι, κ, λ, μ, ν, ξ, ο, o, or π papillomavirus.
7. The polypeptide composition of claim 1 , wherein the HPV is a cutaneous HPV.
8. The polypeptide composition of claim 1 , wherein the HPV is a mucosal high risk HPV.
9. The polypeptide composition of claim 1 , wherein the immunogenic peptides are selected from one or more of HPV6, HPV16, HPV18, HPV31, HPV39, HPV51, HPV56, and/or HPV73 poly peptide.
10. The polypeptide composition of claim 1 , wherein the immunogenic peptides are selected from HPV1, HPV5, HPV6, HPV16, and/or HPV18 polypeptide.
11. The polypeptide composition of claim 1 , wherein the immunogenic peptides are selected from one or more of HPV6, HPV16, or HPV18 peptide.
12. The polypeptide composition of claim 1 , wherein the HPV L2 peptide segments correspond to amino acid position 13-45, 17-36, 1-88, 11-88, or 11-200 of SEQ ID NO:1.
13. The polypeptide composition of claim 1 , wherein at least one HPV L2 peptide is a HPV16 L2 peptide.
14. The polypeptide composition of claim 1 , wherein at least one HPV L2 peptide is a HPV18 L2 peptide.
15. The polypeptide composition of claim 1 , wherein at least one HPV L2 peptide is a HPV6 L2 peptide.
16. The polypeptide composition of claim 1 , wherein at least one HPV L2 peptide is a HPV45 L2 peptide.
17. The polypeptide composition of claim 1 , comprising at least 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22 or more immunogenic peptides.
18. The polypeptide composition of claim 1 , comprising at least five immunogenic peptides.
19. The polypeptide composition of claim 1 , comprising at least twenty immunogenic peptides.
20. The polypeptide composition of claim 1 , wherein at least one HPV L2 peptide is a HPV16 L2 peptide.
21. The polypeptide composition of claim 1 , wherein at least one HPV L2 peptide is a HPV18 L2 peptide.
22. The polypeptide composition of claim 1 , wherein at least one HPV L2 peptide is a HPV6 L2 peptide.
23. The polypeptide composition of claim 1 , wherein at least one HPV L2 peptide is a HPV45 L2 peptide.
24. The polypeptide composition of claim 1 , wherein a first HPV L2 peptide is a HPV16 L2 peptide and a second HPV L2 peptide is a HPV18 L2 peptide.
25. The polypeptide composition of claim 1 , wherein a first HPV L2 peptide is a HPV16 L2 peptide and a second HPV L2 peptide is a HPV6 L2 peptide.
26. The polypeptide composition of claim 1 , wherein a first HPV L2 peptide is a HPV18 L2 peptide and a second HPV L2 peptide is a HPV6 L2 peptide.
27. The polypeptide composition of claim 1 , wherein a first HPV L2 peptide is a HPV16 L2, a second HPV L2 peptide is a HPV18 L2 peptide and a third HPV L2 peptide is a HPV6 L2 peptide.
28. The polypeptide composition of claim 1 , wherein the HPV L2 peptide has an amino acid sequence selected from SEQ ID NOs:71-92, and/or SEQ ID NOs:94-106 and/or SEQ ID NOs:110-112.
29. The polypeptide composition of claim 1 , further comprising a non-HPV L2 peptide.
30. The polypeptide composition of claim 29 , wherein the non-HPV L2 peptide is a HPV L1 peptide or HPV L1 protein.
31. The polypeptide composition of claim 29 , wherein the non-HPV L2 peptide is a Th activating epitope, a carrier protein, or an adjuvant.
32. A kit comprising a polypeptide composition of claim 1 .
33. An isolated polypeptide composition comprising at least two homologous papillomavirus L2 immunogenic peptides from at least two isolates of papilloma virus wherein a first immunogenic peptide from a first isolate of papilloma virus is coupled to a second homologous immunogenic peptide from a second isolate of papilloma virus, wherein the polypeptide is selected from the group consisting of SEQ ID NO:93 or SEQ ID NO:107 or SEQ ID NO:108 SEQ ID NO:109 SEQ ID NO:113 and SEQ ID NO:114.