Bacterium producing 2-deoxy-scyllo-inosose (DOI) and method of producing 2-deoxy-scyllo-inosose (DOI) by using same
Disclosed is an Escherichia coli producing 2-deoxy-scyllo-inosose (DOI), which, from a sucrose non-PTS gene group, has at least a sucrose hydrolase (CscA)-encoding gene and which is provided with a DOI production system or has an enhanced DOI production system. The Escherichia coli preferably further includes a system to enhance sugar uptake capacity. There is also disclosed a method of producing DOI from a plant-derived raw material containing sucrose by using the Escherichia coli.
1. A 2-deoxy-scyllo-inosose (DOI)-producing Escherichia coli , which is provided with a DOI production system or has an enhanced DOI production system by introducing a DOI synthase (BtrC)-encoding gene derived from Bacillus circulans into a host, wherein a phosphoglucose isomerase (Pgi)-encoding gene and a glucose-6-phosphate-1-dehydrogenase (Zwf)-encoding gene of the host are disrupted, and
only a sucrose hydrolase (CscA)-encoding gene derived from Escherichia coli from the gene group consisting of a repressor protein (CscR)-encoding gene, a sucrose hydrolase (CscA)-encoding gene, a fructokinase (CscK)-encoding gene, and a sucrose permease (CscB)-encoding gene is introduced into the Escherichia coli to provide only CscA activity from the group consisting of CscR activity, CscA activity, CscK activity, and CscB activity.
2. The DOI-producing Escherichia coli according to claim 1 , wherein the sucrose hydrolase (CscA)-encoding gene is derived from Escherichia coli O 157 bacteria.
3. The DOI-producing Escherichia coli according to claim 1 , further comprising an enhanced glucose transport facilitator activity by introducing a glucose transport facilitator (Glf) gene derived from bacteria belonging to the genus Zymomonas.
4. The DOI-producing Escherichia coli according to claim 3 , wherein the bacteria belonging to the genus Zymomonas is Zymomonas mobilis bacteria.
5. The DOI-producing Escherichia coli according to claim 1 , which is a B strain or a derivative thereof.
6. A method of producing DOI, from a plant-derived raw material containing sucrose by culturing the DOI-producing Escherichia coli according to claim 1 in a medium comprising the plant-derived raw material containing sucrose.