Scaffold-kinase interaction blockades and uses thereof in treating cancer
View Patent ↗Aspects of the invention include compositions and methods for inhibiting the interaction between scaffold proteins and kinases. These compositions and methods find a number of uses including, for example, suppressing tumor growth and metastasis and reducing tumor size and number in a mammal with cancer.
1. A method of inhibiting RAS pathway activity in a cell, the method comprising:
adding to a cell in vitro an effective amount of an IQ motif containing GTPase activating protein I (IQGAP1) scaffold-kinase interaction blockade (SKIB) agent to block the interaction of a scaffold protein and a kinase thereby inhibiting RAS pathway activity in the cell, wherein the SKIB agent is an isolated IQGAP1 WW peptide or a nucleic acid encoding an isolated IQGAP1 WW peptide, wherein the IQGAP1 WW peptide consists essentially of the WW domain of IQGAP1.
2. The method according to claim 1 , wherein the cell is a cancer cell, and the cancer is a RAS pathway-driven cancer.
3. The method according to claim 2 , wherein the cell is selected from the group consisting of a skin cancer cell, a breast cancer cell, a colorectal cancer cell, and a prostate cancer cell.
4. The method according to claim 2 , wherein the method inhibits RAS-driven cell proliferation and/or cell metastasis.