Hydrogels with covalently linked polypeptides
The present invention provides methods of synthesizing hydrogels that contain covalently linked polypeptides. These polypeptides may be tetravalent peptides or polypeptides that bind to cell surface receptors. The hydrogels synthesized by the methods of the present invention may be used in wound dressings or applied directly to wounds to promote healing.
1. A method of synthesizing a hydrogel comprising:
(a) reacting a C-terminal cysteine sulfhydryl group of a polypeptide with a halogen on a compound represented by formula (I):
wherein n is an integer between 1 and 6, R 1 represents a halogen, and R 2 represents a methyl, an ethyl, a hydroxymethyl, a hydroxyethyl, or a hydrogen; and
(b) adding the reacted polypeptide to an acrylic acid derivative with a radical initiator to begin polymerization of the hydrogel.
2. The method of claim 1 , wherein the polypeptide is incorporated into a tetravalent structure.
3. The method of claim 2 , wherein the polypeptide is selected from the group consisting of WNSTL (SEQ ID NO: 2), NQHTPR (SEQ ID NO: 3), VSNQH (SEQ ID NO: 4), and VQATQS (SEQ ID NO: 5).
4. The method of claim 1 , wherein the polypeptide binds to cell surface receptors.
5. The method of claim 4 , wherein the polypeptide is a full-length polypeptide or an active peptide fragment of a polypeptide selected from the group consisting of cytokines, growth factors, cell adhesion molecules, and antibodies.
6. The method of claim 1 , wherein the polypeptide has a molecular weight of less than 200 kilodaltons (kD).
7. The method of claim 1 , wherein the compound represented by formula (I) is selected from the group consisting of 3-iodo-2-methyl-1-propene and allyl-iodide.
8. The method of claim 1 , wherein the acrylic acid derivative is selected from the group consisting of acrylic acid, 2-hydroxyethyl methacrylate, methyl methacrylate, 2-acrylamido-2-methylpropane sulfonic acid (AMPS), and hydroxymethacrylate.
9. The method of claim 1 , further comprising:
(c) adding a pharmacologically-active agent to the hydrogel after polymerization is terminated.
10. The method of claim 9 , wherein the pharmacologically-active agent is selected from the group consisting of vulnerary agents, hemostatic agents, antibiotics, antihelminthics, anti-fungal agents, hormones, anti-inflammatory agents, polypeptides, oligonucleotides, cytokines, antibodies, and enzymes.
11. A hydrogel synthesized with the method of claim 1 .
12. A wound dressing comprising the hydrogel of claim 11 .
13. A method of treating a wound in a subject comprising applying to the wound the hydrogel of claim 11 .