Measuring circulating therapeutic antibody, antigen and antigen/antibody complexes using ELISA assays
The present invention relates to the field of immunology and hyperproliferative diseases. More specifically, the present invention relates to a method of detecting and monitoring therapeutic antibody:antigen complex, soluble antigen and soluble therapeutic antibody, wherein a patient has undergone at least one course of immunotherapy. Yet further, levels of therapeutic antibody:antigen complexes, soluble antigens or soluble therapeutic antibodies may be measured and used to stage or monitor a hyperproliferative disease.
1. A method of monitoring the progress of therapy in patient being treated with a therapeutic anti-CD33 antibody, the method comprising testing a sample from the patient to measure the level of circulating cell-free CD33, circulating cell-free anti-CD33 or circulating cell-free CD33/anti-CD33 complexes.
2. The method of claim 1 , wherein the sample is selected from the group consisting of serum, cell lysate and plasma.
3. The method of claim 1 , wherein the patient is being treated with Mylotarg.
4. The method of claim 1 , wherein the sample is tested to measure the level of circulating cell-free CD33.
5. The method of claim 1 , wherein the sample is tested to measure the level of circulating cell-free anti-CD33.
6. The method of claim 1 , wherein the sample is tested to measure the level of circulating cell-free CD33/anti-CD33 complexes.