High sensitivity quantitation of peptides by mass spectrometry
The instant invention provides an economical flow-through method for determining amount of target proteins in a sample. An antibody preparation (whether polyclonal or monoclonal, or any equivalent specific binding agent) is used to capture and thus enrich a specific monitor peptide (a specific peptide fragment of a protein to be quantitated in a proteolytic digest of a complex protein sample) and an internal standard peptide (the same chemical structure but including stable isotope labels). Upon elution into a suitable mass spectrometer, the natural (sample derived) and internal standard (isotope labeled) peptides are quantitated, and their measured abundance ratio used to calculate the abundance of the monitor peptide, and its parent protein, in the initial sample.
1. A method for quantifying the amount of a protein in a bodily fluid, comprising:
contacting a sample comprising a proteolytic digest of said bodily fluid and a labeled reference peptide with an anti-peptide antibody, wherein said anti-peptide antibody specifically binds a preselected peptide in said digest and said reference peptide;
separating peptides bound by said antibody from unbound peptides,
eluting peptides bound by said antibody from said antibody;
measuring by mass spectrometry the amount of said preselected peptide and said reference peptide eluted from said antibody;
and calculating the amount of said protein in said bodily fluid.
2. The method of claim 1 , wherein said labeled reference peptide includes at least one site at which a stable isotope of different mass is substituted for the predominant natural isotope in more than 98% of peptide molecules.
3. The method of claim 1 , further comprising: creating the anti-peptide antibody using the peptide or a non-materially modified version of the peptide.
4. The method of claim 1 , wherein said bound peptides are subjected to a chromatography step after elution from said antibody and before introduction into said mass spectrometer.
5. The method of claim 1 , wherein the anti-peptide antibody is a polyclonal antibody.
6. The method of claim 1 , wherein the anti-peptide antibody is a monoclonal antibody.
7. The method of claim 5 , wherein the polyclonal antibody is created using said preselected peptide or a non-materially modified version of said preselected peptide.
8. The method of claim 1 wherein said bodily fluid is a human bodily fluid.
9. The method of claim 1 wherein said bodily fluid is blood serum.
10. The method of claim 1 wherein said bodily fluid is blood plasma.
11. The method of claim 8 , wherein said bodily fluid is blood serum.
12. The method of claim 8 , wherein said bodily fluid is blood plasma.
13. The method of claim 1 wherein said reference peptide is prepared by proteolysis of a larger polypeptide or protein.