IP Library Granted Patent US 9,173,902
Granted Patent B2
US 9,173,902 · App. 12/530,617 · Granted Nov 3, 2015

Skin-whitening agent

Inventors: Tatsuya Watanabe (Fuchu, JP); Ken Kato (Kawagoe, JP); Hiroshi Ueno (Kawagoe, JP); Yuko Haruta (Kawagoe, JP); Noriko Ueda (Kawagoe, JP); Toshimitsu Yoshioka (Kawagoe, JP)
Assignee: MEGMILK SNOW BRAND CO., LTD.
A61K35/20A23C9/1526A23K1/1631A23K1/1846A23L1/3053A23L2/52A61K8/64A61K38/018A61Q19/02A61K2800/92
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 9,173,902
App. No.
12/530,617
Granted
Nov 3, 2015
Kind
B2
Abstract

A skin-whitening agent offering an excellent whitening effect and being effective in preventing and treating age spots, freckles, etc., where such skin-whitening agent contains as its effective ingredient a whey protein hydrolyzate characterized by a molecular weight distribution of 10 kDa or less, main peak of 200 Da to 3 kDa, APL (average peptide-chain length) of 2 to 8, free amino acid content of 20% or less, and antigenicity of one-ten thousandth of β-lactoglobulin or less, as well as a cosmetic, drink or food, feed, pharmaceutical preparation or other skin-whitening product containing such skin-whitening agent.

Claims (11)

1. A method for skin-whitening, comprising:

providing a whey protein hydrolyzate having the following characteristics:

(A) the molecular weight is distributed within 10 kDa with a main peak of 200 Da to 500 Da;

(B) the APL (average peptide-chain length) is 2 to 8;

(C) the free amino acid content is 20% or less; and

(D) the antigenicity is one-ten thousandth of antigenicity of β-lactoglobulin or less; and

orally administrating the whey protein hydrolyzate as a skin-whitening agent to a subject desiring skin-whitening, in an amount of at least 5 mg of the whey protein hydrolyzate per day,

wherein the step of providing the whey protein hydrolyzate comprises:

(i) enzymatically decomposing a whey protein as a preliminary decomposition using a protein hydrolyzing enzyme under pH 6 to 10 at 20 to 55° C., and then raising the temperature for further decomposition of the whey protein in step (ii); and

(ii) enzymatically decomposing the hydrolyzed whey protein obtained in step (i) while thermally denaturing the hydrolyzed whey protein using a heat-resistant protein hydrolyzing enzyme under pH 6 to 10 at 50 to 70° C. until a main peak of the hydrolyzed whey protein is reduced to 200 Da to 500 Da as measured when concentrated using a molecular weight cut off in a range of 2 kDa to 10 kDa and in a range of 150 Da to 300 Da, and then heating the obtained product and thereby deactivating the enzyme.

2. The method according to claim 1 , wherein the whey protein hydrolyzate is administrated to the subject at a concentration of 0.01 to 1%.

Assignments (3)
CORRECTIVE ASSIGNMENT TO CORRECT THE NATURE OF CONVEYANCE PREVIOUSLY RECORDED ON REEL 027495 FRAME 0616. ASSIGNOR(S) HEREBY CONFIRMS THE NATURE OF CONVEYANCE IS AN ABSORPTION AND MERGER OF ASSIGNOR INTO ASSIGNEE, AND NOT SIMPLY A CHANGE OF NAME. Recorded Feb 2, 2012
From: SNOW BRAND MILK PRODUCTS CO., LTD.
To: MEGMILK SNOW BRAND CO., LTD.
Reel/Frame 027646/0183 →
CHANGE OF NAME Recorded Jan 6, 2012
From: SNOW BRAND MILK PRODUCTS CO., LTD.
To: MEGMILK SNOW BRAND CO., LTD.
Reel/Frame 027495/0616 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 3, 2010
From: WATANABE, TATSUYA; KATO, KEN; UENO, HIROSHI; HARUTA, YUKO; UEDA, NORIKO; YOSHIOKA, TOSHIMITSU
To: SNOW BRAND MILK PRODUCTS CO., LTD.
Reel/Frame 023894/0464 →
Priority Claims (2)
JP 2007-063053 · Mar 13, 2007 · national
JP 2007-299516 · Nov 19, 2007 · national
Continuity (1)
Related Publication 20100135941A1 · Jun 3, 2010