Apparatus and methods for conducting assays and high throughput screening
The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.
1. A method of conducting cellular analysis, comprising
(a) providing a suspension of cells,
(b) labeling at least a portion of the cells with a labeled ligand,
(c) introducing the cells into a channel configured and arranged to deliver a plurality of individual cells from the suspension into a mass spectrometer,
(d) measuring the label from the plurality of individual cells by mass spectrometry to determine a property of the individual cells.
2. The method of claim 1 wherein the ligand is an antibody.
3. The method of claim 2 wherein the label is a mass label.
4. The method of claim 1 wherein the property is a cell surface protein.
5. The method of claim 1 wherein the property is expression of a protein marker by the cell.
6. The method of claim 2 wherein the property is expression of a protein marker by the cell.
7. The method of claim 3 wherein the property is expression of a protein marker by the cell.
8. The method of claim 1 wherein the cells are eukaryotic cells.
9. The method of claim 2 wherein the cells are eukaryotic cells.
10. The method of claim 3 wherein the cells are eukaryotic cells.
11. The method of claim 4 wherein the cells are eukaryotic cells.
12. The method of claim 7 wherein the cells are eukaryotic cells.
13. The method of claim 1 wherein the cells alive and are metabolically functioning.
14. The method of claim 8 wherein the cells alive and are metabolically functioning.
15. The method of claim 10 wherein the cells alive and are metabolically functioning.
16. The method of claim 11 wherein the cells alive and are metabolically functioning.
17. The method of claim 12 wherein the cells alive and are metabolically functioning.