Mass spectrometric quantitation assay for metabolites of leflunomide
View Patent ↗Methods are described for determining the amount of metabolites of leflunomide in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying teriflunomide in a sample.
1. A method for determining the amount of teriflunomide in a sample by mass spectrometry, said method comprising:
a. subjecting the sample to ionization in positive ion mode under conditions suitable to produce one or more ions detectable by mass spectrometry;
b. determining the amount of one or more ions by mass spectrometry, wherein said one or more ions determined by mass spectrometry are selected from the group consisting of ions with mass to charge ratios (m/z) of 271.3 ±0.50, 162.1 ±0.50, and 142.2 ±0.50; and
c. using the amount of the one or more ions determined in step (b) to determine the amount of teriflunomide in the sample.
2. The method of claim 1 , wherein ionization is conducted with an electrospray ionization (ESI) source.
3. The method of claim 1 , wherein the sample comprises a biological sample.
4. The method of claim 1 , wherein the sample comprises plasma or serum.
5. The method of claim 1 , wherein the sample is subjected to protein precipitation prior to mass spectrometric ionization.
6. The method of claim 1 , wherein the method is capable of detecting teriflunomide at levels within the range of about 2.5 ng/mL to about 5000 ng/mL, inclusive.
7. The method of claim 1 , wherein the method is capable of detecting teriflunomide at levels within the range of about 10 ng/mL to about 5000 ng/mL, inclusive.
8. The method of claim 1 , wherein the sample is subjected to liquid chromatography prior to ionization.
9. The method of claim 8 , wherein said liquid chromatography comprises high performance liquid chromatography.
10. The method of claim 1 , wherein said mass spectrometry is tandem mass spectrometry.
11. The method of claim 10 , wherein tandem mass spectrometry is conducted by multiple reaction monitoring, precursor ion scanning, or product ion scanning.
12. The method of claim 10 , wherein said tandem mass spectrometry comprises fragmenting a precursor ion with a mass to charge ratio (m/z) of 271.3 ±0.50 into one or more fragment ions selected from the group consisting of ions with m/z of 162.1 ±0.50 and 142.2 ±0.50.
13. The method of claim 12 , wherein the amount of a fragment ion with m/z of 162.1 ±0.50 is used to determine the amount of teriflunomide in a sample.
14. The method of claim 12 , wherein the ratio of the amount of a fragment ion with m/z of 162.1 ±0.50 to the amount of a fragment ion with m/z of 142.2 ±0.50 is used to confirm the identity of teriflunomide.
15. A method for performing a cholestyramine drug elimination procedure, the method comprising:
a) administering cholestyramine to a patient;
b) obtaining a plasma or serum sample from the patient, and
c) detecting the amount of teriflunomide in the plasma or serum sample by the method of claim 1 .
16. The method of claim 15 , wherein if the amount of teriflunomide present in the plasma or serum sample is less than or equal to 20 ng/mL, the cholestyramine drug elimination procedure was effective.
17. The method of claim 15 , wherein if the amount of teriflunomide present in the plasma or serum sample is greater than 20 ng/mL, the method further comprises repeating steps a)-c).
18. The method of claim 15 , wherein if the amount of teriflunomide present in the plasma or serum sample is less than or equal to 20 ng/mL, the method further comprises repeating step c) to confirm the first result.
19. The method of claim 18 , wherein if the results of two teriflunomide determinations indicate that teriflunomide is present at levels of less than or equal to 20 ng/mL, the cholestyramine drug elimination procedure was effective.