Simian subfamily B adenoviruses SAdV-28, -27, -29, -32, -33, and -35 and uses thereof
A recombinant vector comprises simian adenovirus 28, simian adenovirus 27, simian adenovirus 32, simian adenovirus 33, and/or simian adenovirus 35 sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses one or more simian adenovirus-28, -27, -32, -33, or -35 genes is also disclosed. Methods of using the vectors and cell lines are provided.
1. An adenovirus having a capsid comprising a hexon protein, a penton protein, and a fiber protein, wherein said hexon protein is the hexon protein of SAdV-27, amino acids 1 to 956 of SEQ ID NO: 49; said capsid encapsidating a heterologous nucleic acid comprising a gene operably linked to expression control sequences which direct transcription, translation, and/or expression thereof in a host cell.
2. The adenovirus according to claim 1 , further comprising a 5′ and a 3′ adenovirus cis-element necessary for replication and encapsidation.
3. The adenovirus according to claim 1 , wherein said adenovirus lacks all or a part of the E1 gene.
4. The adenovirus according to claim 3 , wherein said adenovirus is replication-defective.
5. The adenovirus according to claim 1 , wherein said adenovirus has a hybrid capsid.
6. The adenovirus according to claim 5 , wherein said hybrid capsid comprises at least one capsid protein from one or more adenoviruses selected from SAdV-28, SAdV-29, SAdV-32, SAdV-33, and SAdV-35.
7. The adenovirus according to claim 1 , wherein said adenovirus is a pseudotyped adenovirus.
8. The adenovirus according to claim 1 , wherein said adenovirus comprises one or more adenovirus genes.
9. A composition comprising a virus according to claim 1 in a pharmaceutically acceptable carrier.
10. A composition comprising the adenovirus according to claim 1 , further comprising one or more simian adenovirus proteins selected from the group consisting of:
E1a, SEQ ID NO:68;
E1b, small T/19K, SEQ ID NO:61;
E1b, large T/55K, SEQ ID NO:40;
IX, SEQ ID NO:41;
52/55D, SEQ ID NO:42;
IIIa, SEQ ID NO:43;
Penton, SEQ ID NO:44;
VII, SEQ ID NO:45;
V, SEQ ID NO:46;
pX, SEQ ID NO:47;
VI, SEQ ID NO:48;
Endoprotease, SEQ ID NO:50;
100 kD, SEQ ID NO:51;
33 kD, SEQ ID NO:70;
22 kD, SEQ ID NO:63;
VIII, SEQ ID NO:52;
E3/12.5 K, SEQ ID NO:53;
CR1-alpha, SEQ ID NO:64;
gp19K, SEQ ID NO:54;
CR1-beta, SEQ ID NO:55;
CR1-gamma, SEQ ID NO:56;
CR1-delta, SEQ ID NO:57;
RID-alpha, SEQ ID NO:65;
RID-beta, SEQ ID NO:58;
E3/14.7K, SEQ ID NO:66; and
Fiber, SEQ ID NO:59.
11. The adenovirus according to claim 1 , wherein said penton protein is the penton protein of SAdV-28, amino acids 1 to 582 of SEQ ID NO: 6; the penton protein of SAdV-27, amino acids 1 to 562 of SEQ ID NO: 44; the penton protein of SAdV-29, amino acids 1 to 576 of SEQ ID NO: 76; the penton protein of SAdV-32, amino acids 1 to 585of SEQ ID NO: 108; the penton protein of SAdV-33, amino acids 1 to 571 of SEQ ID NO: 139; or the penton protein of SAdV-35, amino acids 1 to 563 of SEQ ID NO: 171.
12. The adenovirus according to claim 1 , wherein said fiber protein is the fiber protein of SAdV-28, amino acids 1 to 323 of SEQ ID NO: 21, the fiber protein of SAdV-27, amino acids 1 to 325 of SEQ ID NO: 59; the fiber protein of SAdV-29, amino acids 1 to 324 of SEQ ID NO: 91; the fiber protein of SAdV-32, amino acids 1 to 319 of SEQ ID NO: 123; the fiber protein of SAdV-33, amino acids 1 to 325 of SEQ ID NO: 154; or the fiber protein of SAdV-35, amino acids 1 to 353 of SEQ ID NO: 185.