Device and method for removing a supernatant of a liquid sample, and use of a valve device having a soluble membrane
A device and a method for separating a supernatant of a liquid sample are proposed, in which an outlet is closed off by a water-soluble membrane that dissolves after a certain length of time so that the supernatant of the sample is automatically drained away after centrifugation. The water-soluble membrane may in principle may also be used for other purposes for temporarily retaining a liquid in a holding chamber.
1. A device ( 1 ) for removing a supernatant of a liquid sample ( 2 ), comprising:
a holding chamber ( 4 ) for the sample ( 2 ) having a proximal end and a distal end separated from one another along a longitudinal axis,
a retaining region ( 9 ) disposed at the distal end of the holding chamber ( 4 ) and operating to collect constituents of the liquid sample that migrate towards the distal end of the holding chamber ( 4 ) during centrifugation,
an outlet ( 5 ) having an entrance port and an exit port separated from one another along the longitudinal axis, the entrance port being located upstream from the retaining region ( 9 ) along the longitudinal axis such that the supernatant may flow into the entrance port while the constituents of the liquid sample remain within the retaining region ( 9 ), and
a valve device ( 11 ) associated with the outlet ( 5 ) such that: (i) when the valve ( 11 ) is closed, the supernatant is blocked from flowing into the entrance port of the outlet ( 5 ); and (ii) when the valve ( 11 ) is open, the supernatant is permitted to flow into the entrance port of the outlet ( 5 ),
wherein the retaining region ( 9 ), outlet ( 5 ) and valve ( 11 ) are fixed in position along the longitudinal axis within the holding chamber ( 4 ).
2. The device according to claim 1 , wherein the entrance port of the outlet ( 5 ) is located upstream from the retaining region ( 9 ) along the longitudinal axis such that when the valve device ( 11 ) is open the supernatant of the sample ( 2 ) drains out of the holding chamber ( 4 ) only upstream of the retaining region ( 9 ).
3. The device according to claim 1 , wherein the valve device ( 11 ) opens automatically.
4. The device according to claim 1 , wherein the valve device ( 11 ) comprises a water-soluble membrane ( 12 ).
5. The device according to claim 1 , wherein the valve device ( 11 ) opens or bursts when at least one of a predetermined pressure is exceeded, and after a predetermined time.
6. The device according to claim 1 , wherein the device ( 1 ) comprises a collecting chamber ( 6 ) into which the exit of the outlet ( 5 ) communicates for the supernatant.
7. The device according to claim 6 , wherein the device ( 1 ) comprises an upper part ( 7 ) and a lower part ( 8 ), the holding chamber ( 4 ) being arranged in the upper part ( 7 ) and the collecting chamber ( 6 ) being arranged in the lower part ( 8 ), and the upper part ( 7 ) and the lower part ( 8 ) are at least one of releasable from one another, and releasably connectable to one another.
8. The device according to claim 1 , wherein the device ( 1 ) comprises at least one abutment ( 13 ) for mounting in a centrifuge.
9. The device according to claim 1 , wherein the valve device ( 11 ) comprises a soluble membrane ( 12 ) for temporarily retaining the liquid ( 2 ) in the holding chamber ( 4 ), and the valve device ( 11 ) opens automatically as a result of the membrane ( 12 ) being at least partially dissolved by the liquid ( 2 ).