Inhibition of bolting and flowering of a sugar beet plant
A means for inhibiting the bolting and flowering of sugar beet plant, including an isolated nucleic acid, a vector or mobile genetic element which can be used to produce a sugar beet, in which stem elongation post-vernalization is inhibited. Further, a transgenic sugar beet plant comprising the nucleic acid, vector or mobile genetic element, in which bolting and flowering is inhibited after vernalization, a method for producing such transgenic sugar beet plant, and a process for the inhibition of bolting and flowering of sugar beet plant.
1. An isolated nucleic acid for the inhibition of bolting and flowering of sugar beet plant, wherein the nucleic acid comprises a nucleotide sequence which
a) comprises SEQ ID NO:5, 6 or 7 or at least 1183 consecutive nucleotides of SEQ ID NO: 2 or 3, or
b) is complementary to SEQ ID NO:5, 6 or 7 or at least 1183 consecutive nucleotides of SEQ ID NO: 2 or 3, or
c) comprises in the antisense direction SEQ ID NO:5, 6 or 7 or at least 1183 consecutive nucleotides of SEQ ID NO: 2 or 3 or a complementary sequence thereof, or
d) comprises SEQ ID NO: 5, 6 or 7 and is at least 90% identical to a sequence selected from the group consisting of SEQ ID NO: 2-3, or comprises SEQ ID NO: 5, 6 or 7 and is at least 90% identical to a partial sequence of a sequence selected from the group consisting of SEQ ID NO: 2-3, or
e) comprises SEQ ID NO: 5, 6 or 7 and encodes a protein with the amino acid sequence of SEQ ID NO: 4 or a part of the protein comprising at least 101 consecutive amino acids of SEQ ID NO: 4, or
f) comprises SEQ ID NO: 5, 6 or 7 and encodes a protein with an amino acid sequence which is at least 90% identical to the sequence of SEQ ID NO: 4 or at least 90% identical to a sequence a sequence segment comprising at least 101 consecutive amino acids of SEQ ID NO: 4, or
g) comprises SEQ ID NO: 5, 6 or 7 and hybridizes under stringent conditions with a sequence selected from the group consisting of SEQ ID NO: 2-3 or a nucleotide sequence complementary thereto or a nucleotide sequence oriented in the antisense direction thereto, wherein the stringent conditions include hybridization in 4×SSC at 65° C., followed by repeated washing in 0.1×SSC at 65° C. for a total of about 1 hour.
2. The nucleic acid according to claim 1 , wherein the nucleic acid is at least 95% identical to a sequence or partial sequence of SEQ ID NO: 2-3.
3. A method for producing a transgenic sugar beet plant, where the bolting and flowering is inhibited after vernalization, comprising the steps of
(a) transforming a sugar beet cell with one or more nucleic acids according to claim 1 and (b) generating a sugar beet plant from the transformed sugar beet cell.
4. A vector or mobile genetic element, comprising one or more nucleic acids according to claim 1 and/or a nucleic acid for the inhibition of bolting and flowering of a sugar beet plant, wherein the nucleic acid comprises a nucleotide sequence which
a) is a partial sequence comprising at least 303 consecutive nucleotides of SEQ ID NO: 2 or 3, or b) is complementary to a partial sequence comprising at least 303 consecutive nucleotides of SEQ ID NO: 2 or 3, or exhibits in the antisense direction a partial sequence comprising at least 303 consecutive nucleotides of SEQ ID NO: 2 or 3 or a complementary sequence thereof.
5. A transgenic sugar beet plant in which bolting and flowering is inhibited, wherein the transgenic sugar beet plant comprises one or more nucleic acids according to claim 1 or the vector or mobile genetic element according to claim 4 as transgenes.
6. A seed or part of a transgenic sugar beet plant in which bolting and flowering is inhibited, which is transformed with one or more nucleic acids according to claim 1 or the vector or mobile genetic element according to claim 4 as transgenes.
7. A process for the inhibition of bolting and flowering of sugar beet plant comprising the steps of (a) introducing one or more nucleic acids according to claim 1 in a sugar beet cell, and (b) generating a sugar beet plant from the sugar beet cell, wherein the introduced one or more nucleic acids leads to a reduction of the protein transcript having an amino acid sequence of SEQ ID NO: 4 or of a thereto homologous protein from Beta vulgaris in the sugar beet plant.
8. The nucleic acid of claim 1 , wherein the sequence comprising SEQ ID NO:5, 6 or 7 comprises at least 350 consecutive nucleotides of SEQ ID NO: 2 or 3, and/or the part of the protein comprises at least 101 consecutive amino acids of SEQ ID NO: 4, and/or the protein comprises at least 150 sequential amino acids of SEQ ID NO: 4.
9. The nucleic acid of claim 1 , wherein the sequence sequence comprising SEQ ID NO:5, 6 or 7 comprises at least 450 consecutive nucleotides of SEQ ID NO: 2 or 3, and/or the the protein comprises at least 250 sequential amino acids of SEQ ID NO: 4.
10. The nucleic acid according to claim 1 , wherein the nucleic acid is at least 97% identical to a sequence or partial sequence of SEQ ID NO: 2-3.
11. The nucleic acid according to claim 1 , wherein the nucleic acid is at least 99.5% identical to a sequence or partial sequence of SEQ ID NO: 2-3.
12. The nucleic acid according to claim 1 , wherein the nucleic acid is at least 99.6% identical to a sequence or partial sequence of SEQ ID NO: 2-3.
13. The nucleic acid according to claim 1 , wherein the nucleic acid is at least 99.7% identical to a sequence or partial sequence of SEQ ID NO: 2-3.
14. The nucleic acid according to claim 1 , wherein the nucleic acid is at least 99.8% identical to a sequence or partial sequence of SEQ ID NO: 2-3.
15. The nucleic acid according to claim 1 , wherein the nucleic acid is at least 99.9% identical to a sequence or partial sequence of SEQ ID NO: 2-3.