Multi-hierarchical self-assembly of a collagen mimetic peptide
The present disclosure generally relates to collagen, and more particularly compositions and methods related to collagen-mimetic peptides. More specifically, the present disclosure provides a collagen-mimetic peptide and peptide systems comprising the amino acid sequence (Pro-Lys-Gly) 4 (Pro-Hyp-Gly) 4 (Asp-Hyp-Gly) 4 .
1. A collagen-mimetic peptide of SEQ. ID. NO. 1.
2. A collagen-mimetic hydrogel comprising a peptide, the peptide comprising the sequence of SEQ. ID. NO. 1.
3. The hydrogel of claim 2 , wherein the hydrogel is biocompatible.
4. The hydrogel of claim 2 , wherein the hydrogel is viscoelastic.
5. A method of preparing a collagen-mimetic hydrogel comprising:
obtaining a plurality of collagen-mimetic peptides, wherein each of the plurality of collagen-mimetic peptides comprise the peptide sequence of SEQ ID NO: 1;
placing the plurality of collagen-mimetic peptides in a buffer; and
allowing the plurality of collagen-mimetic peptides to self-assemble to form the collagen-mimetic hydrogel.
6. The method of claim 5 , wherein allowing the peptides to self-assemble to form a hydrogel comprises allowing the peptides to self-assemble to form a plurality of triple helices.
7. The method of claim 6 , wherein allowing the peptides to self-assemble to form a hydrogel comprises allowing the triple helices to self-assemble to form a plurality of fibers.
8. The method of claim 7 , wherein allowing the peptides to self-assemble to form a hydrogel comprises allowing the plurality of fibers to self-assemble to form a hydrogel network.
9. The method of claim 5 , wherein preparing a plurality of collagen-mimetic peptides comprises solid phase peptide synthesis.
10. The method of claim 5 wherein the buffer is sodium phosphate, water, Tris, or PBS.
11. The method of claim 5 , wherein the plurality of collagen-mimetic peptides are placed in the buffer at a concentration of from about 0.5% to about 2% by weight.
12. The method of claim 5 , wherein the buffer is of a pH of about 7.
13. A biomaterial comprising a collagen-mimetic hydrogel, wherein the hydrogel comprises a peptide comprising the sequence of SEQ. ID. NO. 1.
14. The biomaterial of claim 13 , wherein the biomaterial is a scaffold for tissue engineering applications.
15. The biomaterial of claim 13 , wherein the biomaterial is biocompatible.