Multisample bionanochip platform
A bionanochip cartridge for analysis of multiple samples or analytes is provided herein, and the cartridge is dimensioned to take advantage of existing robotic microtiter plate handling equipment. Fluidics are specially designed to provide a small footprint and to prevent cross contamination.
1. A bionanochip cartridge comprising:
a) a substrate having an exterior footprint the width and length and shape of a standard microtiter plate; and,
b) said substrate having a plurality of sample analysis arrangements therein, each sample analysis arrangement having:
i) a top loading inlet port fluidly connected to a reaction chamber that is laterally spaced from said inlet port;
ii) an indirect fluid pathway connecting said inlet port and said reaction chamber;
iii) said reaction chamber having a transparent cover overhead allowing visual inspection of said reaction chamber from overhead;
iv) said reaction chamber having a porous base; and,
v) said reaction chamber fluidly connected under said porous base to an outlet port.
2. The bionanochip cartridge of claim 1 , wherein said indirect fluidic pathway has 4 changes of direction before reaching said reaction chamber.
3. The bionanochip cartridge of claim 1 , wherein said indirect fluidic pathway has 3 changes of direction before reaching said reaction chamber.
4. The bionanochip cartridge of claim 1 , wherein said indirect fluidic pathway has 2 or 1 changes of direction before reaching said reaction chamber.
5. The bionanochip cartridge of claim 1 , wherein said indirect fluidic pathway begins at a top of said cartridge, proceeds to below said reaction chamber, then laterally, then to above said reaction chamber, then laterally, and then down to said reaction chamber.
6. The bionanochip cartridge of claim 1 , further comprising a plurality of outlet pathways beneath said membrane, said outlet pathways fluidly connected to said outlet port.
7. The bionanochip cartridge of claim 1 , wherein said porous base comprises a membrane.
8. The bionanochip cartridge of claim 1 , wherein said porous base comprises a membrane and a porous frit beneath said membrane.
9. The bionanochip cartridge of claim 1 , wherein said fluidic pathway has a hydrophilic fluid contacting surface.
10. The bionanochip cartridge of claim 1 , further comprising an absorbent pad below said substrate and fluidly connected to said outlet port.
11. The bionanochip cartridge of claim 1 , wherein said outlet port is directly below said reaction chamber.
12. The bionanochip cartridge of claim 1 , wherein said substrate is comprised of at least a transparent cover layer over an inlet layer over a reaction chamber layer over an outlet layer, each of said layers affixed to an adjacent layer in a fluid tight manner.
13. The bionanochip cartridge of claim 1 , wherein said substrate is comprised of at least an inlet layer over a transparent cover layer over a reaction chamber layer over an outlet layer, each of said layers affixed to an adjacent layer in a fluid tight manner.
14. The bionanochip cartridge of claim 1 , wherein said substrate is comprised of at least an inlet layer, a transparent cover layer, a reaction chamber layer, and an outlet layer, each of said layers affixed to an adjacent layer in a fluid tight manner.
15. The bionanochip cartridge of claim 14 , wherein said transparent cover layer is an uppermost layer.
16. The bionanochip cartridge of claim 14 , wherein said transparent cover layer is an intermediate layer.
17. The bionanochip cartridge of claim 14 , wherein at least one of said layers contains at least one pocket on a top or bottom surface thereof for tightly receiving additional layers.
18. The bionanochip cartridge of claim 14 , wherein an uppermost layer has an exterior the size and shape of a microtiter plate and has a pocket on a bottom surface thereof for tightly holding additional layers.
19. The bionanochip cartridge of claim 14 , wherein a middle layer has an exterior the size and shape of a microtiter plate and has pockets on a bottom surface thereof and a top surface thereof for tightly holding additional layers.
20. The bionanochip cartridge of claim 19 , said middle layer having one or more pockets to receive one or more frits.
21. The bionanochip cartridge of claim 1 , further comprising a bottom layer comprising an absorbent material.
22. The bionanochip cartridge of claim 14 , further comprising a bottom layer comprising an absorbent material sized to absorb all fluid from an assay.
23. The bionanochip cartridge of claim 14 , further comprising an impermeable layer above a bottom layer comprising an absorbent material and wherein a hole is provided through said bionanochip cartridge such a that a user can penetrate said impermeable layer through said hole.
24. A bionanochip cartridge comprising:
a) a substrate made of layers held in leak proof juxtaposition and having an exterior footprint the width and length and shape of a standard microtiter plate; and,
b) said substrate having a plurality of sample analysis arrangements therein, each sample analysis arrangement having:
i) a top loading inlet port fluidly connected to a reaction chamber that is laterally spaced from said inlet port;
ii) an indirect fluidic pathway having at least 2 changes of direction connecting said inlet port and said reaction chamber;
iii) said reaction chamber having a transparent cover overhead allowing visual inspection of said reaction chamber from overhead;
iv) said reaction chamber having a porous base;
v) said porous base fluidly connected to a outlet port; and,
vi) an absorbent pad below said outlet port.
25. The bionanochip cartridge of claim 24 , further comprising a plurality of outlet pathways beneath said membrane, said outlet pathways fluidly connected to said outlet port.
26. The bionanochip cartridge of claim 24 , said porous base comprising a membrane for catching cells or a membrane for catching cells with a fit thereunder.
27. The bionanochip cartridge of claim 24 , said porous base comprising an agarose pad or agarose bead.
28. The bionanochip cartridge of claim 27 , said agarose having one or more antibodies conjugated thereto.
29. The bionanochip cartridge of claim 24 , wherein an uppermost layer has an exterior the size and shape of a microtiter plate and has a pocket on a bottom surface thereof for tightly holding additional layers.
30. The bionanochip cartridge of claim 24 , wherein a middle layer has an exterior the size and shape of a microtiter plate and has a bottom pocket on a bottom surface thereof and a top pocket on a top surface thereof for tightly holding additional layers.
31. The bionanochip cartridge of claim 24 , the plurality of sample analysis arrangements reflecting an arrangement of wells on a standard microtiter plate.