Therapeutic and cosmetic uses and applications of calreticulin
View Patent ↗The invention relates to therapeutic and cosmetic uses of calreticulin including reducing or eliminating wrinkles and/or fine lines, tissue repair and reconstruction, repairing damaged bone and/or cartilage, stimulating regeneration of an epidermal appendage, enhancing phagocytosis of bacteria by phagocytes within a wound, treating a wound in a patient suffering from delayed wound healing, treating a corneal wound, and treating or preventing a surgical adhesion.
1. A method of treating wrinkles and/or fine lines in a subject in need thereof, which method comprises administering intradermally to said wrinkles and/or fine lines an amount of calreticulin, which amount is sufficient to increase the production of collagen and/or fibronectin and/or elastin by dermal fibroblasts of the subject.
2. The method of claim 1 , comprising administering said calreticulin in an amount ranging between about 0.001 milligram and about 100 grams.
3. The method of claim 2 , comprising administering said calreticulin in an amount ranging between about 0.01 milligram and about 50 milligrams.
4. The method of claim 1 , further comprising administering a compound selected from the group consisting of a cytokine, a growth factor, collagen, a glycosaminoglycan, a proteoglycan, and syndecan, or any mixtures thereof.
5. The method of claim 4 , wherein said glycosaminoglycan is hyaluronic acid.
6. The method of claim 4 , wherein said proteoglycan is perlecan or heparin sulfate.
7. The method of claim 4 , wherein said growth factor is selected from the group consisting of a platelet-derived growth factor, vascular endothelial growth factor, fibroblast growth factor, epidermal growth factor, transforming growth factor-beta, and any mixtures thereof.
8. A method of treating wrinkles and/or fine lines in a subject in need thereof, which method comprises administering to said wrinkles and/or fine lines a peptide consisting of N—, P— or C-domain of calreticulin in an amount sufficient to increase the production of collagen and/or fibronectin and/or elastin by dermal fibroblasts of the subject.
9. The method of claim 8 , comprising administering said peptide subcutaneously, intradermally, or transdermally.
10. The method of claim 8 , comprising administering said peptide in an amount ranging between about 0.001 milligram and about 100 grams.
11. The method of claim 10 , comprising administering said peptide in an amount ranging between about 0.01 milligram and about 50 milligrams.
12. The method of claim 8 , further comprising administering a compound selected from the group consisting of a cytokine, a growth factor, collagen, a glycosaminoglycan, a proteoglycan, and syndecan, or any mixtures thereof.
13. The method of claim 12 , wherein said glycosaminoglycan is hyaluronic acid.
14. The method of claim 12 , wherein said proteoglycan is perlecan or heparin sulfate.
15. The method of claim 12 , wherein said growth factor is selected from the group consisting of a platelet-derived growth factor, vascular endothelial growth factor, fibroblast growth factor, epidermal growth factor, transforming growth factor-beta, and any mixtures thereof.