Cellular arrays and methods of detecting and using genetic disorder markers
A method is disclosed for rapid molecular profiling of tissue or other cellular specimens by placing a donor specimen in an assigned location in a recipient array, providing copies of the array, and performing a different biological analysis of each copy. The results of the different biological analyses are compared to determine if there are correlations between the results of the different biological analyses at each assigned location. In some embodiments, the specimens may be tissue specimens from different tumors, which are subjected to multiple parallel molecular (including genetic and immunological) analyses. The results of the parallel analyses are then used to detect common molecular characteristics of the genetic disorder type, which can subsequently be used in the diagnosis or treatment of the disease. The biological characteristics of the tissue can be correlated with clinical or other information, to detect characteristics associated with the tissue, such as susceptibility or resistance to particular types of drug treatment. Other examples of suitable tissues which can be placed in the matrix include tissue from transgenic or model organisms, or cellular suspensions (such as cytological preparations or specimens of liquid malignancies or cell lines).
1. A method of detecting the presence of cancerous cells in a human breast tissue specimen, the method comprising:
a) obtaining a breast tissue specimen,
b) providing a probe set consisting of two or more labeled probes selected from a locus specific probe for chromosomal region 7q31, a locus specific probe for chromosomal region 8p11-p12, a locus specific probe for chromosomal region 10q25 and a probe specific for PDGFB, wherein at least one probe is specific for PDGFB,
c) contacting said specimen with said two or more labeled probes of b), and
d) detecting the presence of cancerous cells when PDGFB and at least one of the chromosomal regions of step b) are amplified in the human breast tissue specimen.
2. The method of claim 1 , wherein said 10q25 amplification is detected by polymerase chain reaction (PCR).
3. The method of claim 1 , wherein said 10q25 amplification is detected by fluorescence in situ hybridization (FISH).
4. The method of claim 1 , wherein polymerase chain reaction (PCR) is used to determine if amplification is present.
5. The method of claim 1 , wherein fluorescence in situ hybridization (FISH) is used to determine if amplification is present.
6. The method of claim 1 , wherein a cDNA microarray is used to determine if amplification is present.