Use of SLIM-1 in the assessment of heart failure
The invention relates to a method for assessing heart failure in vitro comprising the steps of measuring in a sample the concentration of the marker SLIM-1, of optionally measuring in the sample the concentration of one or more other marker(s) of heart failure, and of assessing heart failure by comparing the concentration determined in for SLIM-1 and the concentration(s) determined for the optionally one or more other marker to the concentration of this marker or these markers as established in a reference population. Also disclosed are the use of SLIM-1 as a marker protein in the assessment of heart failure, a marker combination comprising SLIM-1 and a kit for measuring SLIM-1.
1. A method of detecting an amount of four and a half LIM domain-1 (SLIM-1) in a subject, the method comprising:
contacting a portion of a sample from the subject with a detectable antibody having specific binding affinity for SLIM-1 and wherein the antibody binds within amino acids 233-246 of SEQ ID NO:1, thereby forming a detectable complex between the detectable antibody and any SLIM-1 present in the sample, wherein the sample is selected from the group consisting of serum, plasma and blood;
separating the detectable complex from uncomplexed antibody; and
quantifying a signal from the detectable complex, the signal being proportional to an amount of SLIM-1 in the sample, thereby detecting an amount of SLIM-1 in the sample.
2. The method of claim 1 , further comprising determining an amount of at least one additional marker in a portion of the sample from the subject.
3. The method of claim 2 , wherein the additional marker is selected from the group consisting of troponin T, NT-proBNP, and CRP.