Compositions, comprising improved IL-12 genetic constructs and vaccines, immunotherapeutics and methods of using the same
Nucleic acid molecules and compositions comprising: a nucleic acid sequence that encodes IL-12 p35 subunit or a functional fragment thereof and/or a nucleic acid sequence that encodes IL-12 p40 subunit or a functional fragment thereof, are disclosed. The nucleic acid molecules and compositions further comprising a nucleic acid sequence that encodes an immunogen are also disclosed. Method of modulating immune response and methods of inducing an immune response against an immunogen are disclosed. Therapeutic and prophylactic vaccination methods are also disclosed.
1. A composition that comprises a) a nucleic acid sequence that encodes IL-12 p35 subunit and b) a nucleic acid sequence that encodes IL-12 p40 subunit, wherein
the nucleic acid sequence that encodes IL-12 p35 subunit is at least 98% homologous to SEQ ID NO:1 and encodes a protein at least 98% homologous to SEQ ID NO:2, and
the nucleic acid sequence that encodes IL-12 p40 subunit is at least 98% homologous to SEQ ID NO:3 and encodes a protein at least 98% homologous to SEQ ID NO:4.
2. The composition of claim 1 comprising
the nucleic acid sequence that encodes IL-12 p35 subunit is at least 98% homologous to SEQ ID NO:1 and encodes a protein at least 99% homologous to SEQ ID NO:2, and
the nucleic acid sequence that encodes IL-12 p40 subunit is at least 98% homologous to SEQ ID NO:3 and encodes a protein at least 99% homologous to SEQ ID NO:4.
3. The composition of claim 2 comprising the nucleic acid sequence that encodes IL-12 p35 subunit is at least 98% homologous to SEQ ID NO:1 and encodes SEQ ID NO:2, and the nucleic acid sequence that encodes IL-12 p40 subunit is at least 98% homologous to SEQ ID NO:3 and encodes SEQ ID NO:4.
4. The composition of claim 1 comprising
the nucleic acid sequence that encodes IL-12 p35 subunit is at least 99% homologous to SEQ ID NO:1 and encodes a protein at least 99% homologous to SEQ ID NO:2, and
the nucleic acid sequence that encodes IL-12 p40 subunit is at least 99% homologous to SEQ ID NO:3 and encodes a protein at least 99% homologous to SEQ ID NO:4.
5. The composition of claim 1 comprising
the nucleic acid sequence that encodes IL-12 p35 subunit is at least 99% homologous to SEQ ID NO:1 and encodes SEQ ID NO:2, and
the nucleic acid sequence that encodes IL-12 p40 subunit is at least 99% homologous to SEQ ID NO:3 and encodes SEQ ID NO:4.
6. The composition of claim 1 comprising
the nucleic acid sequence that encodes IL-12 p35 subunit is SEQ ID NO:1, and
the nucleic acid sequence that encodes IL-12 p40 subunit is SEQ ID NO:3.
7. The composition of claim 1 formulated for delivery to an individual using electroporation.
8. The composition of claim 1 wherein the nucleic acid sequence that encodes IL-12 p35 subunit is on a different nucleic acid molecule than the nucleic acid sequence that encodes IL-12 p40 subunit.
9. The composition of claim 1 wherein the nucleic acid sequence that encodes IL-12 p35 subunit is on a plasmid and the nucleic acid sequence that encodes IL-12 p40 subunit is on a different plasmid.
10. The composition of claim 1 wherein the nucleic acid sequence that encodes IL-12 p35 subunit and the nucleic acid sequence that encodes IL-12 p40 subunit are on the same nucleic acid molecule.
11. The composition of claim 1 wherein the nucleic acid sequence that encodes IL-12 p35 subunit and the nucleic acid sequence that encodes IL-12 p40 subunit are on the same plasmid.
12. The composition of claim 1 wherein the nucleic acid sequence that encodes IL-12 p35 subunit and the nucleic acid sequence that encodes IL-12 p40 subunit are on the same nucleic acid molecule and operably linked to different promoters.
13. The composition of claim 1 wherein the nucleic acid sequence that encodes IL-12 p35 subunit and the nucleic acid sequence that encodes IL-12 p 40 subunit are on the same plasmid and operably linked to different promoters.
14. The composition of claim 1 further comprising a nucleic acid sequence that encodes an immunogen.
15. The composition of claim 1 further comprising a nucleic acid sequence that encodes an immunogen from a pathogen selected from the group consisting of:
HIV, HPV, HCV, Influenza, Smallpox, Chikungunya, foot and mouth disease virus, Malaria, human cytomegalovirus, human respiratory syncytial virus, and MRSA.
16. The composition of claim 1 wherein the nucleic acid sequence that encodes IL-12 p35 subunit and the nucleic acid sequence that encodes IL-12 p40 subunit are incorporated into a viral particle.
17. The composition of claim 1 further comprising a nucleic acid sequence that encodes one or more proteins selected from the group consisting of: IL-15 and IL-28.
18. The composition of claim 1 comprising a) the nucleic acid sequence that encodes the IL-12 p35 subunit that is free of the coding sequence of IL-12 p35 subunit signal peptide and is linked to a nucleic acid sequence that encodes a non-IL12 p35 signal peptide and b) the nucleic acid sequence that encodes the IL-12 p40 subunit that is free of the coding sequence of IL-12 p40 subunit signal peptide and is linked to a nucleic acid sequence that encodes a non-IL12 p40 signal peptide.
19. The composition of claim 18 comprising a) the nucleic acid sequence that encodes the IL-12 p35 subunit that is free of the coding sequence of IL-12 p35 subunit signal peptide and linked to a nucleic acid sequence that encodes SEQ ID NO:5 and b) the nucleic acid sequence that encodes the IL-12 p35 subunit that is free of the coding sequence of IL-12 p40 subunit signal peptide and linked to a nucleic acid sequence that encodes SEQ ID NO:5.
20. A method of inducing an immune response against an immunogen comprising administering to an individual, a composition of claim 1 in combination with a nucleic acid sequence that encodes an immunogen in an amount effective to induce an immune response in said individual.
21. The method of claim 20 wherein the composition further comprises a nucleic acid sequence that encodes an immunogen.