Compositions and methods useful for the treatment and diagnosis of inflammatory bowel disease
Compositions and methods useful for the diagnosis and treatment of IBD including Crohn's disease are disclosed.
1. A kit for practicing a method of determining response to therapy in a human subject having Inflammatory Bowel Disease (IBD) that includes measuring in a serum sample from the subject the level of at least one differentially expressed miRNA, wherein the differentially expressed miRNA comprises miR-484 having a serum miRNA significance threshold of p<0.05,
said kit comprising an oligonucleotide that specifically hybridizes to at least one differentially expressed miRNA, said oligonucleotide being (i) immobilized on a solid support or (ii) detectably labeled, and one or more of a physiologically acceptable carrier, instructions for use, a container for serum, and an assay substrate;
wherein said at least one differentially expressed miRNA comprises miR-484.
2. The kit of claim 1 , wherein said kit comprises an internal reference miRNA that is one or both of miR-150 and miR-342-3p.
3. The kit of claim 1 , wherein the differentially expressed miRNA comprises at least two miRNA selected from the group consisting of miR-195, miR-16, miR-93, miR-140, miR-30e, miR-20a, miR-106a, miR-192, let-7b, miR-21, and miR-484.
4. The kit of claim 1 , wherein the differentially expressed miRNA comprises at least five miRNA selected from the group consisting of miR-195, miR-16, miR-93, miR-140, miR-30e, miR-20a, miR-106a, miR-192, let-7b, miR-21, and miR-484.
5. The kit of claim 1 , wherein the differentially expressed miRNA comprises at least ten miRNA selected from the group consisting of miR-195, miR-16, miR-93, miR-140, miR-30e, miR-20a, miR-106a, miR-192, let-7b, miR-21, and miR-484.
6. The kit of claim 1 , wherein the differentially expressed miRNA comprises miR-195, miR-16, miR-93, miR-140, miR-30e, miR-20a, miR-106a, miR-192, let-7b, miR-21, and miR-484.