Spatial frequency swept interference illumination
Spatial frequency swept interference (SFSI) illumination and imaging methods and devices that interfere two collimated coherent beams to generate an interference pattern of a plurality of illuminating sheets with sweeping spatial frequency.
1. A spatial frequency swept interference illumination device, comprising,
a spatial light modulator for displaying two interlaced diffraction patterns; and
an optical system for transforming the displayed interlaced diffraction patterns into a plurality of illumination sheets with sweeping spatial frequency.
2. The spatial frequency swept interference illumination device of claim 1 , wherein the optical system comprises:
a first lens for projecting two moving spots to a Fourier plane, each moving spot based on one of the two interlaced diffraction patterns; and
a second lens for reverse Fourier transforming the two spots into two intersecting collimated coherent beams providing an interference pattern of a plurality of illumination sheets with varying spatial frequency.
3. The spatial frequency swept interference illumination device of claim 2 , wherein the optical system is a 4-f system.
4. The spatial frequency swept interference illumination device of claim 2 , further comprising a slit aperture at the Fourier plane.
5. The spatial frequency swept interference illumination device of claim 1 , wherein the optical system transforms the displayed interlaced diffraction patterns into two intersecting collimated coherent beams providing an interference pattern comprising the plurality of illumination sheets with varying spatial frequency.
6. The spatial frequency swept interference illumination device of claim 5 , wherein the spatial light modulator is also for modifying a relative phase of the intersecting collimated coherent beams.
7. The spatial frequency swept interference illumination device of claim 1 , wherein each of the interlaced diffraction patterns is a blazed grating.
8. A method of spatial frequency swept interference illumination, comprising:
sweeping spatial frequency of a plurality of illumination sheets from an interference pattern generated by two intersecting collimated coherent beams; and
changing a relative phase of the two intersecting collimated coherent beams to two or more different phases.
9. The method of spatial frequency swept interference illumination of claim 8 , further comprising:
capturing frames of a sample illuminated by the plurality of illumination sheets at different relative phases;
generating a 3D complex dataset based on the captured frames based on different relative phases; and
reverse Fourier transforming the 3D complex dataset into a spatial domain to generate a 3D image of the sample.
10. The method of spatial frequency swept interference illumination of claim 8 , further comprising capturing frames of a sample illuminated by the plurality of illumination sheets at different relative phases.
11. The method of spatial frequency swept interference illumination of claim 10 , further comprising:
generating a complex dataset based on the captured frames based on different relative phases; and
reverse Fourier transforming the complex dataset into a spatial domain to generate an image of the sample.