Cellular arrays and methods of detecting and using genetic disorder markers
A method is disclosed for rapid molecular profiling of tissue or other cellular specimens by placing a donor specimen in an assigned location in a recipient array, providing copies of the array, and performing a different biological analysis of each copy. The results of the different biological analyses are compared to determine if there are correlations between the results of the different biological analyses at each assigned location. In some embodiments, the specimens may be tissue specimens from different tumors, which are subjected to multiple parallel molecular (including genetic and immunological) analyses. The results of the parallel analyses are then used to detect common molecular characteristic of the genetic disorder type, which can subsequently be used in the diagnosis or treatment of the disease. The biological characteristics of the tissue can be correlated with clinical or other information, to detect characteristics associated with the tissue.
1. A composition suitable for detecting the presence of cancerous cells in a human breast tissue specimen, said composition comprising nucleic acids, wherein said nucleic acids consist of (i) a labeled probe suitable for detecting expression of PDGF-β, said probe obtained by PCR screening of a human genomic library and suitable for detecting expression of PDGF- βin the human breast cancer specimen; (ii) a labeled locus specific probe for chromosomal region 7q31; (iii) a labeled locus specific probe for chromosomal region 8p11-p12; and (iv) a labeled locus specific probe for chromosomal region 10q25-26, wherein probes (ii), (iii), and (iv) are suitable for detecting their target chromosomal regions in the human breast cancer specimen, and wherein probes (i), (ii), (iii), and (iv) are labeled with a fluorescent dye.
2. The composition of claim 1 used in a method to detect breast cancer, wherein probes (i), (ii), (iii), and (iv) are detected by polymerase chain reaction (PCR).
3. The composition of claim 1 used in a method to detect breast cancer, wherein probes (i), (ii), (iii), and (iv) are detected by fluorescence in situ hybridization (FISH).
4. The composition of claim 1 used in a method to detect breast cancer, wherein probes (i), (ii), (iii), and (iv) are detected using a cDNA microarray.
5. The composition of claim 1 wherein probes (i), (ii), (iii), and (iv) are each labeled with fluorescent dye of a unique emission wave length.
6. A composition suitable for detecting the presence of cancerous cells in a human breast tissue specimen, said composition comprising nucleic acids, wherein said nucleic acids consist of (i) a labeled probe suitable for detecting expression of PDGF-β, said probe obtained by PCR screening of a human genomic library and suitable for detecting expression of PDGF-βin the human breast cancer specimen; (ii) a labeled locus specific probe for chromosomal region 7q31; and (iii) a labeled locus specific probe for chromosomal region 8p11-p12, wherein probes (ii) and (iii) are suitable for detecting their target chromosomal regions in the human breast cancer specimen, and wherein probes (i), (ii), and (iii) are labeled with a fluorescent dye.
7. A composition suitable for detecting the presence of cancerous cells in a human breast tissue specimen, said composition comprising nucleic acids, wherein said nucleic acids consist of (i) a labeled probe suitable for detecting expression of PDGF-β, said probe obtained by PCR screening of a human genomic library and suitable for detecting expression of PDGF-βin the human breast cancer specimen; (ii) a labeled locus specific probe for chromosomal region 7q31; and (iii) a labeled locus specific probe for chromosomal region 10q25-26, wherein probes (ii) and (iii) are suitable for detecting their target chromosomal regions in the human breast cancer specimen, and wherein probes (i), (ii), and (iii) are labeled with a fluorescent dye.