Composition and method for delivery of living cells in a dry mode having a surface layer
The present invention generally relates to compositions and methods of delivering living cells in a dry mode, wherein the compositions include a surface layer disposed on the outer surface of the composition that is permeable to carbon dioxide and oxygen. The compositions may be used to deliver living cells to a delivery point without the use of expensive refrigerants such as dry ice or liquid nitrogen.
1. A composition for delivering living cells in a dry mode, the composition comprising:
an inert carrier substrate having pores;
living cells loaded within the pores of the inert carrier substrate; and
a surface layer disposed on an outer surface of the inert carrier substrate loaded with the living cells, wherein the surface layer is permeable to molecules that aid in cell growth of the living cells such that the composition is operable to allow for increased propagation of the living cells within the inert carrier substrate as compared to another composition having an absence of the surface layer.
2. The composition as claimed in claim 1 , wherein the molecules that permeate the surface layer include oxygen and carbon dioxide.
3. The composition as claimed in claim 1 , wherein the inert carrier substrate is selected from the group consisting of diatomaceous earth, walnut and pecan shells, rice hulls, cellulosic clay, montmorillonite clay, bentonite clay, wool, cotton, cellulose, corn cobs, cellulose shells, precipitated silica, and combinations thereof.
4. The composition as claimed in claim 1 , wherein the inert carrier substrate is precipitated silica.
5. The composition as claimed in claim 1 , wherein the surface layer comprises an organic phase.
6. The composition as claimed in claim 5 , wherein the organic phase comprises a fatty acid.
7. The composition as claimed in claim 6 , wherein the fatty acid is an unsaturated fatty acid.
8. The composition as claimed in claim 1 , wherein the surface layer is substantially impermeable to water.
9. The composition as claimed in claim 1 , wherein the surface layer is substantially insoluble to deionized water.
10. The composition as claimed in claim 1 , wherein the surface layer is susceptible to surfactants, oil, organic solvents, salt water, damp soil, or combinations thereof.
11. The composition as claimed in claim 1 , wherein the surface layer is at least partially soluble to surfactants, oil, organic solvents, salt water, damp soil, or combinations thereof.
12. The composition as claimed in claim 1 , wherein the surface layer further comprises an absence of a protein.
13. The composition as claimed in claim 1 , further comprising an absence of zeolites.
14. The composition as claimed in claim 1 , further comprising an absence of aluminosilicates.
15. The composition as claimed in claim 1 , further comprising an absence of a mineral powder.
16. The composition as claimed in claim 1 , wherein the living cells are selected from the group consisting of human cells, primary cells, cell lines, immortalized cells, lymphatic cells, and combinations thereof.
17. The composition as claimed in claim 1 , further comprising an absence of an acidic polymer.
18. The composition as claimed in claim 1 , further comprising nutrients in contact with the living cells, wherein the nutrients are operable to provide a food source to the living cells loaded throughout the inert carrier substrate such that the living cells can propagate.
19. The composition as claimed in claim 18 , wherein the nutrients are selected from the group consisting of ammonia, nitrogen, ammonium nitrogen, urea, dextrose, dextrin, sugars, and combinations thereof.
20. The composition as claimed in claim 1 , wherein the inert carrier substrate has pores that have diameters within the range of 38 to 240 nanometers.
21. The composition as claimed in claim 1 , wherein the composition has an initial living cell count, and the composition is operable to maintain approximately 50 to 400% of the initial living cell count for at least 45 days.
22. A composition for delivering living cells in a dry mode, the composition comprising:
an inert carrier substrate having pores;
a surface layer disposed on an outer surface of the inert carrier substrate loaded with the living cells, wherein the surface layer is permeable to oxygen and carbon dioxide; and
living cells loaded within the pores of the inert carrier substrate, the composition having 25 to 75% living cell by weight, the composition operable to maintain approximately 50 to 100% of the living cell by weight for a period of at least 45 days.
23. The composition as claimed in claim 22 , wherein the composition is substantially dry.
24. The composition as claimed in claim 22 , wherein the composition is not hygroscopic.