Compositions and methods for immunodominant antigens
Contemplated compositions, devices, and methods comprise immunodominant antigens from selected human pathogens ( Burkholderia pseudomallei, Borrelia burgdorferi, Brucella melitensis, Chlamydia muridarum, Coxiella burnetii, Francisella tularensis , human Herpes virus 1 and 2, Mycobacterium tuberculosis, Plasmodium falciparum , and Vaccinia virus) can be used as a vaccine, as diagnostic markers, and as therapeutic agents. In particularly preferred aspects, the antigens have quantified and known relative reactivities with respect to sera of a population infected with the pathogen, and have a known association with a disease parameter.
1. An isolated antigen composition comprising:
a plurality of immunodominant antigens of a pathogenic organism disposed on a solid carrier; and
wherein the plurality of antigens are encoded by nucleic acids selected from the group consisting of SEQ ID NO:503 and SEQ ID NO:512, or fragments thereof.
2. The isolated antigen composition of claim 1 further comprising at least one additional antigen of Brucella melitensis , encoded by nucleic acids selected from the group consisting of SEQ ID NO:446 to SEQ ID NO:545.
3. The isolated antigen composition of claim 1 wherein the plurality of antigens is disposed on the carrier in an array.
4. The isolated antigen composition of claim 3 wherein each of the antigens are present in a purity of greater than 60%.
5. The isolated antigen composition of claim 3 wherein the antigens or fragments thereof are recombinant.
6. The isolated antigen composition of claim 3 wherein the antigens or fragments thereof are purified.