IP Library › Granted Patent US 9,315,586
Granted Patent B2
US 9,315,586 · App. 13/422,722 · Granted Apr 19, 2016

Method and carrier complexes for delivering molecules to cells

Inventors: Hazel Szeto (New York, NY); Kesheng Zhao (Jackson Heights, NY); Hugh Robertson (New York, NY); Alex V. Birk (Woodhaven, NY)
Assignee: Cornell Research Foundation, Inc.
C07K17/00A61K47/48246C07K5/08C07K5/1016C07K5/1019C12N15/87
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Quick Facts
Patent No.
US 9,315,586
App. No.
13/422,722
Granted
Apr 19, 2016
Kind
B2
Abstract

The invention relates to carrier complexes and methods for delivering molecules to cells. The carrier complexes comprises a molecule and an aromatic cationic peptide in accordance with the invention. In one embodiment, the method for delivering a molecule to a cell comprises contacting the cell with a carrier complex. In another embodiment, the method for delivering a molecule to a cell comprises contacting the cell with a molecule and an aromatic cationic peptide.

Claims (20)

1. A method for delivering a molecule to a cell, the method comprising contacting the cell with a carrier complex, wherein the carrier complex comprises the molecule conjugated to an aromatic cationic peptide, wherein the aromatic cationic peptide is selected from the group consisting of: Tyr-D-Arg-Phe-Lys-NH 2 (DALDA), 2′,6′-Dmt-D-Arg-Phe-Lys-NH 2 (Dmt 1 -DALDA), Phe-D-Arg-Phe-Lys-NH 2 (Phe 1 -DALDA), D-Arg-2′,6′-Dmt-Lys-Phe-NH 2 , and 2′,6′-Dmp-D-Arg-Phe-Lys-NH 2 (Dmp 1 -DALDA); and wherein the molecule is a lipid.

2. A method according to claim 1 , wherein the peptide has the formula Tyr-D-Arg-Phe-Lys-NH 2 (DALDA).

3. A method according to claim 1 , wherein the peptide has the formula 2′,6′-Dmt-D-Arg-Phe-Lys-NH 2 (Dmt 1 -DALDA).

4. A method according to claim 1 , wherein the peptide has the formula Phe-D-Arg-Phe-Lys-NH 2 (Phe 1 -DALDA).

5. A method according to claim 1 , wherein the peptide has the formula D-Arg-2′,6′-Dmt-Lys-Phe-NH 2 .

6. A method according to claim 1 , wherein the peptide has the formula 2′,6′-Dmp-D-Arg-Phe-Lys-NH 2 (Dmp 1 -DALDA).

7. A method according to claim 1 , wherein the cell is a bacterial cell.

8. A method according to claim 1 , wherein the cell is a plant cell.

9. A method according to claim 1 , wherein the cell is an animal cell.

10. A method according to claim 9 , wherein the animal cell is a mammalian cell.

11. A method according to claim 9 , wherein the cell is a neuronal cell.

12. A method according to claim 9 , wherein the cell is a renal epithelial cell.

13. A method according to claim 9 , wherein the cell is an intestinal epithelial cell.

14. A method according to claim 9 , wherein the cell is a vascular endothelial cell.

15. A method according to claim 14 , wherein the endothelial cell is a blood-brain barrier endothelial cell.

16. A method according to claim 9 , wherein the cell is a glial cell.

17. A method according to claim 9 , wherein the cell is a hepatocyte.

18. A method according to claim 1 , wherein the aromatic-cationic peptide comprises a linker.

19. A method according to claim 1 , wherein the molecule comprises a linker.

20. A method according to claim 1 , wherein the molecule and aromatic cationic peptide are chemically bonded.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 23, 2012
From: SZETO, HAZEL H.; ZHAO, KESHENG; ROBERTSON, HUGH D.; BIRK, ALEX V.
To: CORNELL RESEARCH FOUNDATION, INC.
Reel/Frame 028090/0159 →
Continuity (4)
Continuation 12631048 · Dec 4, 2009
Continuation 10838135 · May 3, 2004
Provisional Application 60467516 · May 1, 2003
Related Publication 20130085259A1 · Apr 4, 2013