Prompt nucleic acid delivery carrier composition
An object of the present invention is to provide a carrier composition for nucleic acid delivery, which can efficiently deliver a nucleic acid into cells when a nucleic acid such as siRNA is administered to animal-derived cells or animals, and also has low toxicity and high safety, and a composition for nucleic acid delivery containing the carrier composition and nucleic acid. A carrier for nucleic acid delivery is prepared by using (A) a diacylphosphatidylcholine, (B) at least one member selected from the group consisting of cholesterol and derivatives thereof, and (C) an aliphatic primary amine. Also, a composition for nucleic acid delivery is prepared by mixing the carrier for nucleic acid delivery with a nucleic acid.
1. A composition for delivery of siRNA comprising an siRNA, and a carrier composition for delivery of siRNA, consisting essentially of (A) at least one member selected from the group consisting of dimyristoylphosphatidycholine and distearoylphosphatidylcholine , (B) cholesterol , and (C) an aliphatic primary amine, wherein the molar ratio of component (A): component (B): component (C) is 6-9:1-4:1, wherein the total amount of components (A) to (C) contained in the composition for delivery of siRNA is from 30 to 90% by weight, based on the total amount of the composition.
2. The composition for delivery of siRNA according to claim 1 , wherein the component (C) is an alkylamine having 10 to 20 carbon atoms.
3. The composition for delivery of siRNA according to claim 1 ,
wherein the component (A) is at least one member selected from the group consisting of dimyristoylphosphatidylcholine, and distearoylphosphatidylcholine;
the component (B) is cholesterol; and
the component (C) is stearylamine.
4. The composition for delivery of siRNA according to claim 1 , wherein the carrier composition for delivery of siRNA is a liposome preparation in which a liposomal membrane is formed of the components (A) to (C).
5. The composition for delivery of siRNA according to claim 1 , wherein the composition is a liposomal preparation.
6. A method for introducing a siRNA, which comprises the step of introducing the siRNA into cells by bringing the composition for of siRNA delivery of claim 1 into contact with the cells.
7. A method for introducing a siRNA according to claim 6 , wherein the cells are cultured cells, cells separated from living organisms, or cells existing in living organisms.