Systems and methods for identifying and disrupting cellular organelles

1. A method comprising:

(a) directing a target object comprising an organelle to a platform that receives an ablation radiation

(b) photosensitizing the organelle to the ablation radiation;

(c) generating an image of the target object on the platform;

(d) generating an outline of the photosensitized organelle based upon the image; and

(e) emitting the ablation radiation toward the specimen to selectively disrupt the photosensitized organelle wherein the emitting comprises at least one of:

(i) emitting the ablation radiation based on a shape determined by the organelle outline;

(ii) translocating the ablation radiation in a movement pattern determined by the organelle outline; and

(iii) maintaining the ablation radiation substantially stationary while translocating the target object in a movement pattern determined by the organelle outline.

2. The method of claim 1 , wherein directing the target object further comprises flowing the target object through a flow path.

3. The method of claim 2 , wherein flowing the target object through the flow path occurs while emitting the ablation radiation toward the target object.

4. The method of claim 1 , wherein the ablation radiation is emitted by a laser.

5. The method of claim 4 , wherein the laser is a gas laser, a solid-state laser, a tunable dye laser, or semiconductor laser.

6. The method of claim 5 , wherein translocating the ablation radiation comprises scanning by a scanning mirror.

7. The method of claim 1 , wherein the organelle is a nucleus.

8. The method of claim 7 , wherein the target object comprises a stem cell fused to a somatic cell, the stem cell comprising cytoplasm and the nucleus, and the somatic cell comprising a programmed nucleus.

9. The method of claim 8 , wherein emitting the ablation radiation toward the target object to selectively disrupt the sensitized organelle comprises:

inactivating the nucleus of the stem cell such that the stem cell fused to the somatic cell forms a fused cell comprising the cytoplasm of the stem cell and the programmed nucleus of the somatic cell.

10. The method of claim 9 , wherein inactivating the nucleus of the stem cell comprises at least one of inactivating, removing, and destroying a nucleic acid of the nucleus.

11. The method of claim 1 , wherein photosensitizing the organelle comprises tagging the organelle with a light-absorbing molecule.

12. The method of claim 11 , further comprising exciting fluorescence of the light-absorbing molecule while emitting the ablation radiation, wherein the light-absorbing, molecule is a fluorescent molecule excitable by the ablation radiation.

13. The method of claim 1 , further comprising, before emitting the ablation radiation, shielding a portion of the target object from being disrupted.

14. The method of claim 1 , further comprising fusing a stem cell with a somatic cell to form the target object, the target object being a fused cell.

15. The method of claim 1 , wherein the platform further receives a trapping radiation.

16. The method of claim 15 , wherein emitting the ablation radiation toward the target object comprises emitting the ablation radiation sufficient to disrupt the organelle while emitting the trapping radiation sufficient to trap a cell containing said organelle.

17. The method of claim 15 , further comprising photosensitizing the organelle to the trapping radiation.

18. The method of claim 15 , wherein the trapping radiation has a wavelength between 750 nm and 1250 nm, and the ablation radiation has a wavelength between 375 nm and 625 nm.

19. A method comprising:

(a) directing the target object to a platform that receives a first radiation configured to selectively trap the organelle and an ablation second radiation configured to selectively disrupt the organelle;

(b) photosensitizing the organelle to the ablation radiation;

(c) generating an image of the target object on the platform;

(d) generating an outline of the photosensitized organelle in the image;

(e) generating signals corresponding to the organelle by digitizing the organelle outline;

(f) transmitting the signals to an electronic controller operatively associated with an emitter; and

(g) emitting the ablation radiation toward the target object to selectively disrupt the photosensitized organelle.

20. The method of claim 19 , wherein emitting the ablation radiation comprises at least one of:

(i) emitting the ablation radiation in a geometric shape determined by the signals;

(ii) translocating the ablation radiation in a movement pattern determined by the signals; and

(iii) maintaining the ablation radiation substantially stationary while translocating the target object in a movement pattern determined by the signals.