Cell processing apparatus, sample preparation apparatus, and cell analyzer
A cell processing apparatus includes a storage container that contains liquid L including a biological sample; a filter that prevents a first cell C 1 in the biological sample from passing and allows a second cell C 2 having a smaller diameter than that of the first cell C 1 to pass; and a filtration cylinder for separating, in the storage container and via the filter, the liquid L into a first liquid L 1 mainly including the first cell C 1 and a second liquid L 2 mainly including the second cell C 2 . A measurement target cell filtered by the filter among other cells can be easily collected.
1. A sample processing apparatus comprising:
a sample container configured to contain a sample solution including a biological sample collected from a cervix and a preservation solution, the biological sample including epidermal cells of the cervix, red blood cells, white blood cells and bacteria;
a cell disperser configured to disperse the epidermal cells included in the sample solution contained in the sample container;
a filtering unit which includes a first room, a second room and a filter provided between the first room and the second room, wherein the filter is configured to prevent the epidermal cells from passing therethrough and allow the red blood cells, the white blood cells and the bacteria to pass therethrough;
a sample supplier configured to supply, into the first room, the sample solution contained in the sample container;
a pressure generator configured to generate a pressure differential between the first room and the second room such that at least the preservation solution, the red blood cells, the white blood cells and the bacteria are moved from the first room to the second room via the filter;
a substitution liquid supplier configured to supply, into the first room, a substitution liquid which substitutes for the preservation solution in the first room; and
a stirrer that is arranged within the first room and is configured to rotate so as to move the epidermal cells attached to the filter into the substitution liquid in the first room.
2. The sample processing apparatus according to claim 1 , wherein
the pressure generator comprises a negative pressure source configured to apply a negative pressure into the second room.
3. The sample processing apparatus according to claim 2 , wherein
the negative pressure source discharges at least the preservation solution, the red blood cells, the white blood cells and the bacteria which have been moved into the second room via the filter.
4. The sample processing apparatus according to claim 3 , further comprising
a controller configured to control the substitution liquid supplier and the negative pressure source so as to repeat the supply of the substitution liquid and the discharge of at least the preservation solution, the red blood cells, the white blood cells and the bacteria.
5. The sample processing apparatus according to claim 4 , wherein
the controller is configured to control the substitution liquid supplier and the negative pressure source so as to repeat the supply of the substitution liquid and the discharge of at least the preservation solution, the red blood cells, the white blood cells and the bacteria until the preservation solution within the first room is replaced with the substitution liquid.
6. The sample processing apparatus according to claim 1 , wherein
the sample supplier comprises a sample flow path for supplying the sample solution into the first room.
7. The sample processing apparatus according to claim 1 , wherein
the substitution liquid supplier comprises a substitution liquid flow path for supplying the substitution liquid into the first room.
8. The sample processing apparatus according to claim 1 , further comprising
a positive pressure source configured to apply a positive pressure into the second room so as to move the epidermal cells attached to the filter into the substitution liquid in the first room.
9. The sample processing apparatus according to claim 1 , wherein
the stirrer is configured to rotate in a direction along a filtering area of the filter.
10. The sample processing apparatus according to claim 1 , wherein
a distance between the filtering area of the filter and the stirrer opposed to the filtering area is 1mm or less.
11. The sample processing apparatus according to claim 1 , wherein
the filter has a through hole having a diameter of 8 to 20 μm.
12. The sample processing apparatus according to claim 1 , wherein
the filtering unit has a concave on a surface which forms the first room and faces the filter, and the stirrer is arranged within the concave.
13. A sample preparing apparatus comprising:
a sample container configured to contain a sample solution including a biological sample collected from a cervix and a preservation solution, the biological sample including epidermal cells of the cervix, red blood cells, white blood cells and bacteria;
a cell disperser configured to disperse the epidermal cells included in the sample solution contained in the sample container;
a filtering unit which includes a first room, a second room and a filter provided between the first room and the second room, wherein the filter is configured to prevent the epidermal cell from passing therethrough and allow the red blood cells, the white blood cells and the bacteria to pass therethrough;
a sample supplier configured to supply, into the first room, the sample solution contained in the sample storage container;
a pressure generator configured to generate a pressure differential between the first room and the second room such that at least the preservation solution, the red blood cells, the white blood cells and the bacteria are moved from the first room to the second room via the filter;
a substitution liquid supplier configured to supply, into the first room, a substitution liquid which substitutes for the preservation solution in the first room;
a stirrer that is arranged within the first room and is configured to rotate so as to move the epidermal cells attached to the filter into the substitution liquid in the first room; and
a reagent supplier configured to supply a reagent to a liquid taken out from the first room so as to prepare a measurement sample, wherein the liquid taken out from the first room includes the substitution liquid and the epidermal cells.
14. The sample preparing apparatus according to claim 13 , wherein
the reagent is a staining solution for staining the epidermal cells.
15. The sample preparing apparatus according to claim 14 , wherein
the preservation solution contains alcohol.
16. The sample preparing apparatus according to claim 13 , wherein
the pressure generator comprises a negative pressure source configured to apply a negative pressure into the second room.
17. The sample preparing apparatus according to claim 16 , wherein
the negative pressure source discharges at least the preservation solution, the red blood cells, the white blood cells and the bacteria which have been moved into the second room via the filter.
18. The sample preparing apparatus according to claim 17 , further comprising
a controller configured to control the substitution liquid supplier and the negative pressure source so as to repeat the supply of the substitution liquid and the discharge of at least the preservation solution, the red blood cells, the white blood cells and the bacteria.
19. The sample preparing apparatus according to claim 18 , wherein
the controller is configured to control the substitution liquid supplier and the negative pressure source so as to repeat the supply of the substitution liquid and the discharge of the at least the preservation solution, the red blood cells, the white blood cells and the bacteria until the preservation solution within the first room is replaced with the substitution liquid.