Targeted/immunomodulatory fusion proteins and methods for making same
The present invention relates generally to the field of generating fusion proteins to be used in cancer therapy, and more specifically, to nucleotide sequences encoding the fusion proteins, wherein the chimeric fusion proteins comprises at least one targeting moiety and at least one immunomodulatory moiety that counteracts the immune tolerance of cancer cells.
1. A chimeric fusion protein comprising a targeting moiety to target a cancer cell and an immunomodulating moiety that counteracts immune tolerance, wherein the targeting moiety and the immunomodulating moiety are linked by an amino acid spacer selected from SEQ ID NO: 3 or SEQ ID NO: 11, wherein the immunomodulating moiety is TGF-βRII SEQ ID NO: 4, wherein the targeting moiety is Anti-HER2/neu having heavy chain SEQ ID NO: 1 and light chain SEQ ID NO: 2; wherein SEQ ID NO: 4 is attached via the amino acid spacer to the C-terminus of SEQ ID NO: 1 or SEQ ID NO: 2.