Antigen-presenting platelets and methods of eliciting an immune response
Provided herein are methods of producing an antigen-presenting platelet. Also provided herein are methods of eliciting an immune response in a subject using the antigen-presenting platelets described herein. Also provided are methods of screening for an immune response elicited by an antigen-presenting platelet. Further provided are isolated populations of platelets that present a selected antigen produced by the methods described herein.
1. A method of eliciting an immune response in a subject, the method comprising:
a. providing antigen-presenting platelets, wherein the platelets present a selected antigen; and
b. administering to the subject the antigen-presenting platelets, wherein administration of the antigen-presenting platelets elicits an immune response in the subject, wherein the antigen-presenting platelets are of the same species as the subject.
2. The method of claim 1 , wherein the antigen-presenting platelets are administered to the subject at least two times.
3. The method of claim 2 , wherein the second administration of antigen-presenting platelets is at least five, six, seven, eight, nine, ten, eleven, twelve, thirteen, or fourteen days after the first administration.
4. The method of claim 1 , further comprising administering an adjuvant to the subject.
5. The method of claim 1 , wherein the antigen-presenting platelets are produced by platelets isolated from the same subject.
6. The method of claim 1 , wherein the antigen-presenting platelets are produced by platelets isolated from a different subject.
7. The method of claim 1 , wherein the method further comprises administering to the subject an antigen-presenting cell.
8. The method of claim 7 , wherein the antigen-presenting cell is a dendritic cell.