Microbial conversion of sugar acids and means therein
A DNA molecule comprising a fungal gene encoding an enzyme protein capable of converting L-galactonic acid into L-threo-3-deoxy-hexulosonic acid has been cloned and heterologously expressed. The enzyme is involved in the metabolic conversion of sugar acids, which are present in biological waste material such as sugar beet pulp and other pectin comprising material. A microorganism genetically modified to effectively express said enzyme may be used in fermenting biomaterial to desired end products such as ethanol. Alternatively, microorganisms in which the gene has been inactivated may be used to produce L-galactonic acid, which accumulates when the expression of the gene is prevented.
1. A method of producing L-galactonic acid, said method comprising:
providing a fungal microorganism having a gene encoding L-galactonic acid dehydratase capable of converting L-galactonic acid into L-threo-3-deoxy-hexulosonic acid, inactivating said gene by preventing its expression or mutation or deletion of said gene or functional part thereof, and
cultivating the obtained genetically modified micro-organism on a material comprising sugar, sugar acid or derivative thereof and recovering the accumulated L-galactonic acid using the obtained genetically modified microorganism for producing the L-galactonic acid.
2. The method of claim 1 , wherein the microorganism is a microorganism which efficiently utilizes D-galacturonic acid.
3. The method of claim 1 , wherein the genetically modified microorganism is cultivated on biomass.
4. The method of claim 1 , wherein the genetically modified microorganism is cultivated on a substrate comprising D-galacturonic acid or another substrate from which L-galactonic acid can be derived, and the accumulated L-galactonic acid is recovered.
5. The method of claim 4 , wherein the genetically modified microorganism is cultivated on a pectin comprising material.
6. The method of claim 1 , wherein the gene is inactivated by deleting the whole gene or functional part thereof.