Arrangement for determining the concentration of nucleic acids
An arrangement for determining the concentration of nucleic acids in a sample in a microfluidic device. In at least one embodiment, the arrangement having a) a first chamber into which a the sample is introduced, b) an element in the first chamber for carrying out a number of cycles of an amplification reaction to be carried out in cycles for amplifying nucleic acids, c) a second chamber, connectable to the first chamber by way of fluidic communication, equipped with an element to determine concentrations of amplified nucleic acids, d) a control unit to introduce a defined volume of a solution containing reagents for the amplification reaction into the first chamber and to transfer a defined volume from said first chamber to the second chamber.
1. An arrangement, comprising:
a microfluidic device including,
a first chamber with an element for amplifying nucleic acids by means of an amplification reaction to be carried out in cycles,
a second chamber, connectable to the first chamber by way of fluidic communication, equipped with an element to determine concentrations of amplified nucleic acids, and
at least one valve for interrupting and/or releasing fluidic communication between the chambers;
a single pump to transfer nucleic acids; and
a control unit to control the at least one valve and the pump, and to introduce a defined volume of a solution containing reagents for the amplification reaction into the first chamber and to transfer a defined volume from said first chamber to the second chamber, wherein said control unit is connected to the second chamber, said pump and said at least one valve and being responsive to a signal transmitted from said element of the second chamber to said control unit for operating said pump and/or said at least one valve based on the concentration of amplified nucleic acids determined by said element.
2. The arrangement as claimed in claim 1 , wherein the element of the second chamber to determine the concentration includes a microarray having position-specifically immobilized capture oligonucleotides for detecting the amplified nucleic acids.
3. The arrangement as claimed in claim 1 , further comprising at least one electrochemical detection element configured for measuring at least one of currents and potentials.
4. The arrangement as claimed in claim 1 , further comprising one of a heating and a cooling device configured for amplifying nucleic acids by way of an amplification reaction to be carried out in cycles.
5. The arrangement as claimed in claim 4 , wherein the control unit is further designed for providing a temperature profile for the at least one of a heating and cooling device for the first chamber.
6. The arrangement as claimed in claim 1 , further comprising a computer to calculate the concentration of a nucleic acid in a sample on the basis of a signal of the element of the second chamber to determine the concentration.
7. The arrangement as claimed in claim 1 , further comprising at least one reservoir of reagents for determining the concentration of nucleic acids, the reservoir being connectable to the second chamber by way of fluidic communication.
8. The arrangement as claimed in claim 7 , wherein the reservoir comprises an enzyme label.
9. The arrangement as claimed in claim 7 , wherein the reservoir comprises an enzyme substrate.
10. An arrangement, comprising:
a microfluidic device in form of a cartridge including,
a first chamber with an element for amplifying nucleic acids of a sample by means of an amplification reaction to be carried out in cycles,
a second chamber, connectable to the first chamber by way of fluidic communication, equipped with an element to determine concentrations of amplified nucleic acids, the concentration of the amplified nucleic acids are determined using an enzymatic label,
wherein the element of the second chamber is configured for determining the concentrations of the amplified nucleic acids based on an electrochemical signal generated by an enzymatic reaction catalyzed by the enzymatic label,
a channel through which the sample is introduced into the first chamber, and
at least one valve for interrupting and/or releasing fluidic communication between the chambers,
wherein the first chamber contains reagents in dry form carrying out the amplification reaction;
a single pump to transfer nucleic acids; and
a control unit to control the valve and the pump, and to introduce a defined volume of a solution containing reagents for the amplification reaction into the first chamber and to transfer a defined volume from said first chamber to the second chamber,
wherein the arrangement is configured to transfer defined volumes of amplified nucleic acids subsequently to the second chamber based on the electrochemical signal.