Micro-reactor device
View Patent ↗In some aspects, the present disclosure provides a magnetic rack comprising a lateral movement structure and/or a longitudinal movement structure. In particular embodiments, the lateral movement structure and/or longitudinal movement structure move one or more magnets to or away from a reaction tube, in order to control the magnetic forces exerted on microbeads in the reaction tube. The microbeads are attracted onto the reaction tube wall, thereby facilitating separation of the microbeads from a solution in the tube. In particular embodiments, the magnetic rack is used to extract or purify nucleic acid from a sample.
1. A magnetic rack comprising a lateral movement structure and a longitudinal movement structure, wherein:
(1) the lateral movement structure comprises:
a motion platform;
a driving wheel and a driven wheel on the motion platform;
a drive wheel motor connected to the driving wheel;
a timing belt engaging both the driving wheel and the driven wheel; and
a magnet holder engaging the timing belt, which magnet holder is configured to hold one or more magnets,
wherein the timing belt is configured to be actuated by the drive wheel motor and driving wheel to move the magnet holder laterally along a path on the motion platform:
(2) the longitudinal movement structure comprises:
a motor mounting plate;
a lifting motor;
a lifting guide bushing; and
a lifting guide column;
wherein the lifting guide bushing is connected to the motion platform and the lifting guide column is connected to the motor mounting plate,
wherein the lifting guide bushing and the lifting guide column are configured to engage each other to form a lifting structure, and
wherein the lifting motor is configured to actuate the lifting structure to move the motion platform in a direction substantially perpendicular to the motion platform.
2. The magnetic rack of claim 1 , further comprising a photoelectric block on the motion platform.
3. The magnetic rack of claim 1 , wherein the motor mounting plate comprises a photoelectric sensor.
4. The magnetic rack of claim 1 , further comprising a photoelectric block element on the magnet holder and a photoelectric limit element on the motion platform.
5. The magnetic rack of claim 1 , further comprising a fixing belt plate on the timing belt,
wherein the fixing belt plate and the magnet holder are connected or engage each other, and wherein the timing belt actuates the lateral movement of the magnet holder via the fixing belt plate.
6. The magnetic rack of claim 1 , further comprising a mounting seat and a tray for receiving one or more reaction tubes or plates, wherein the tray is configured to be placed on or in the mounting seat.
7. The magnetic rack of claim 6 , wherein the mounting seat houses the lateral movement structure and the longitudinal movement structure.
8. The magnetic rack of claim 1 , further comprising a driven wheel bearing housing on the motion platform.
9. The magnetic rack of claim 1 , wherein the lateral movement structure further comprises a linear guide on the motion platform, wherein the magnet holder is connected to or engages the linear guide, and the timing belt is configured to move the magnet holder laterally along the linear guide.
10. The magnetic rack of claim 1 , further comprising a photoelectric block element on the magnet holder.
11. The magnetic rack of claim 1 , further comprising a photoelectric limit element on the motion platform.
12. The magnetic rack of claim 1 , further comprising a mounting seat that houses the lateral movement structure.
13. The magnetic rack of claim 1 , further comprising a mounting seat that houses the longitudinal movement structure.
14. The magnetic rack of claim 1 , further comprising a tray for receiving one or more reaction tubes or plates, wherein the tray is configured to be placed on or in a mounting seat housing the lateral movement structure or the longitudinal movement structure.
15. A method of performing a magnetic bead-based process, comprising:
placing a receptacle on the magnetic rack of claim 1 , wherein the receptacle contains a sample with a magnetic bead;
actuating the lifting motor of the longitudinal movement structure to move the motion platform comprising one or more magnets in the magnetic holder in a direction substantially perpendicular to the motion platform, whereby the one or more magnets are moved toward the receptacle to attract the magnetic bead onto a side wall of the receptacle; and
actuating the drive wheel motor and driving wheel of the lateral movement structure to move the magnet holder laterally along a path on the motion platform, whereby the one or more magnets are moved toward the bottom of the receptacle to attract the magnetic bead from the side wall onto the bottom of the receptacle.
16. The method of claim 15 , further comprising:
a step of removing or adding a reagent or solution into the receptacle, wherein the magnetic bead remains adsorbed on the side wall of the receptacle when the reagent or solution is being removed or added, and
a step of removing or adding a reagent or solution into the receptacle, wherein the magnetic bead remains adsorbed on the bottom of the receptacle when the reagent or solution is being removed or added.
17. The method of claim 15 , further comprising:
a step of actuating the lifting motor to move the motion platform substantially perpendicular to the motion platform, whereby the one or more magnets are moved away from the receptacle to release the magnetic bead from the side wall of the receptacle; and
a step of actuating the drive wheel motor and driving wheel to move the magnet holder laterally on the motion platform, whereby the one or more magnets are moved away from the receptacle to release the magnetic bead from the bottom of the receptacle.
18. The method of claim 15 , further comprising:
a step of removing or adding a reagent or solution into the receptacle, wherein the magnetic bead remains adsorbed on the side wall of the receptacle when the reagent or solution is being removed or added.
19. The method of claim 15 , further comprising:
a step of removing or adding a reagent or solution into the receptacle, wherein the magnetic bead remains adsorbed on the bottom of the receptacle when the reagent or solution is being removed or added.
20. The method of claim 15 , further comprising:
a step of actuating the lifting motor to move the motion platform substantially perpendicular to the motion platform, whereby the one or more magnets are moved away from the receptacle to release the magnetic bead from the side wall of the receptacle.
21. The method of claim 15 , further comprising:
a step of actuating the drive wheel motor and driving wheel to move the magnet holder laterally on the motion platform, whereby the one or more magnets are moved away from the receptacle to release the magnetic bead from the bottom of the receptacle.