Sample plate for MALDI-TOF mass spectrometer and method of manufacturing the sample plate and mass spectrometry method using the sample plate

1. A sample plate including:

a target plate,

an organic matrix formed on one surface of the target plate,

a parylene thin film formed on the target plate having the organic matrix formed thereon and formed to cover the entire organic matrix, and

a sample fixing layer formed on the parylene thin film,

wherein the sample fixing layer is made of at least one material selected from a group consisting of graphene and carbon nano tube (CNT).

2. The sample plate of claim 1 , wherein the sample fixing layer is preferably made of graphene.

3. The sample plate of claim 1 , at least one material selected from the group consisting of graphene and carbon nano tube (CNT) may be mixed with a sample, which is an analysis target, and a mixture thereof may be formed on the parylene thin film, as the sample fixing layer.

4. The sample plate of claim 1 , the sample fixing layer may be formed on the parylene thin film without being mixed with a sample, which is an analysis target.

5. The sample plate of claim 4 , the sample plate may further include an analysis target formed on the parylene thin film.

6. The sample plate of claim 1 , the parylene thin film is formed to have a thickness of 10 nm to 100 nm.

7. The sample plate of claim 1 , the parylene thin film is a parylene-N thin film.

8. The sample plate of claim 1 , the sample plate is a sample plate for a MALDI-TOF (Matrix Assisted Laser Desorption Ionization Time of Flight) mass spectrometer.

9. A method of manufacturing a sample plate, the method including the steps of:

forming an organic matrix on one surface of a target plate,

forming a parylene thin film on the target plate having the organic matrix formed thereon so that it covers the entire organic matrix, and

forming a sample fixing layer by using at least one material selected from a group consisting of graphene and carbon nano tube (CNT).

10. The method of claim 9 , the step of forming the sample fixing layer may include steps of mixing at least one material selected from the group consisting of graphene and carbon nano tube with a sample, which is an analysis target, and forming a sample fixing layer mixed with the sample on the parylene thin film.

11. The method of claim 10 , the step of forming the sample fixing layer, the sample fixing layer may be formed on the parylene thin film without being mixed with a sample, which is an analysis target.

12. The method of claim 11 , the method may further include a step of forming the sample on the sample fixing layer.

13. The method of claim 10 , the parylene thin film is preferably parylene-N thin film.

14. The method of claim 10 , the step of forming the parylene thin film may include a first step of evaporating parylene dimer, a second step of thermally decomposing the evaporated parylene dimer to form an intermediate product, and a third step of introducing the intermediate product into a vapor deposition chamber and forming the parylene thin film on the target plate having the organic matrix formed thereon.

15. The method of claim 10 , the sample plate is preferably a sample plate for a MALDI-TOF mass spectrometer.

16. A mass spectrometry method using a MALDI-TOF (Matrix Assisted Laser Desorption Ionization Time of Flight) mass spectrometer, the method including the steps of:

providing a sample plate including a target plate, an organic matrix formed on one surface of the target plate, a parylene thin film formed on the target plate having the organic matrix formed thereon and formed to cover the entire organic matrix, and a sample fixing layer formed on the parylene thin film, the sample fixing layer being made of at least one material selected from a group consisting of graphene and carbon nano tube (CNT);

forming a sample, which is an analysis target, on the sample fixing layer;

positioning the sample plate having the sample mounted thereon at a predetermined position of the MALDI-TOF mass spectrometer;

illuminating laser to the sample plate to desorb and ionize the sample, and

performing mass spectrometry for the ionized sample.

17. The method of claim 16 , the material mixed with the sample fixing layer or sample is preferably graphene.

18. A mass spectrometry method using a MALDI-TOF (Matrix Assisted Laser Desorption Ionization Time of Flight) mass spectrometer, the method including the steps of:

providing a sample plate including a target plate, an organic matrix formed on one surface of the target plate, and a parylene thin film formed on the target plate having the organic matrix formed thereon and formed to cover the entire organic matrix; mixing a sample, which is an analysis target, with at least one material selected from a group consisting of graphene and carbon nano tube (CNT);

forming the sample on the sample plate;

positioning the sample plate having the sample mounted thereon at a predetermined position of the MALDI-TOF mass spectrometer;

illuminating laser to the sample plate to desorb and ionize the sample, and

performing mass spectrometry for the ionized sample.

19. The method of claim 18 , the material mixed with a sample fixing layer or sample is preferably graphene.