Electrokinetic device for capturing assayable agents in a dielectric fluid utilizing removable electrodes
Electrokinetic devices and methods are described with the purpose of collecting assayable agents from a dielectric fluid medium. Electrokinetic flow may be induced by the use of plasma generation at high voltage electrodes and consequent transport of charged particles in an electric voltage gradient. In one embodiment, an ionic propulsion device for providing a sample for a bio-specific assay of aerosol particles comprises a housing receiving a sample of aerosol particles and enclosing a high voltage electrode to generate a plasma of electrically charged particles. A carrier assembly is removably receivable in the housing, the carrier assembly comprising a non-conductive carrier and an electrode removably secured to the carrier. Incident to the carrier assembly being received in the housing, the electrode is subject to a voltage so that flow of charged aerosol particles generates a net air flow through the housing and said charged aerosol particles are deposited on the electrode, and said electrode can be removed from said carrier and placed in an extraction vessel for a bio-specific assay.
1. A method for analyzing aerosol particles, comprising:
providing an electrokinetic propulsion device including a housing enclosing a high voltage electrode to generate a plasma of electrically charged particles, and a carrier assembly removably receivable in the housing, the carrier assembly comprising a non-conductive carrier and a capture electrode removably secured to the carrier;
depositing aerosol particles on said capture electrode from a volume of air propelled electrokinetically through said device;
removing said capture electrode from said carrier and placing said capture electrode in an extraction vessel; and
adding a predetermined volume of extraction fluid to said extraction vessel;
agitating said capture electrode in said extraction fluid for a predetermined time,
wherein all or part of said extraction fluid is added to a reaction mixture for analysis of said aerosol particles.
2. The method according to claim 1 wherein removing said capture electrode comprises said carrier assembly comprising a latch slideably received on the carrier to removably secure the capture electrode to the carrier.
3. The method according to claim 1 wherein said carrier assembly engages with the housing of said electrokinetic propulsion device to provide functional alignment of capture electrodes with counter electrode or electrodes within said device.
4. A method for analyzing aerosol particles, comprising:
providing an electrokinetic propulsion device including a housing enclosing a high voltage electrode to generate a plasma of electrically charged particles, and a carrier assembly removably receivable in the housing, the carrier assembly comprising a non-conductive carrier and a capture electrode removably secured to the carrier;
depositing aerosol particles on said capture electrode from a volume of air propelled electrokinetically through said device for a select period of time;
removing said capture electrode from said carrier and extracting the captured aerosol particles from the capture electrode; and
analyzing the captured aerosol particles to determine particle concentration in the volume of air.