Visible light curable hydrogels and methods for using
View Patent ↗This disclosure provides compositions comprising a visible light-curable mixture capable of forming a biocompatible hydrogel, hydrogels prepared from the hydrogel precursor mixtures, and a biocompatible delivery system comprising a hydrogel. The disclosure also provides a process for preparing a multi layer hydrogel delivery system.
1. A composition comprising a visible light-curable mixture capable of forming a biocompatible hydrogel, the mixture comprising one or more macromers, each comprising a strained carbon-carbon multiple bond, one or more cross-linking agents, and one or more type II photoinitiators, each having at least one peak absorbance in the visible light range, wherein the mixture is free of co-initiators.
2. The composition of claim 1 wherein the peak absorbance is in the range from about 400 to about 420 nm, from about 420 to about 480 nm, from about 500 to about 530 nm, or from about 550 to about 570 nm.
3. The composition of claim 1 wherein the mixture has a rapid gel point or a rapid complete gelation time, or a combination thereof.
4. The composition of claim 1 wherein the biocompatible hydrogel exhibits low gel shear modulus or a high gel elastic modulus, or a combination thereof.
5. The composition of claim 1 wherein the mixture further comprises a therapeutic agent, a diagnostic agent, or a population of cells, or a combination thereof.
6. The composition of claim 1 wherein the biocompatible hydrogel is capable of forming a conformal gel coating for immuno-isolation of cells.
7. The composition of claim 1 wherein the photoinitiator is selected from the group consisting of eosins, Rose Bengal, riboflavins, coumarin, and 4-hydroxycoumarin, and combinations thereof.
8. The composition of claim 1 wherein the cross-linking agent is a polythiol compound.
9. The composition of claim 1 wherein the carbon-carbon multiple bond is an alkenyl group.
10. The composition of claim 1 wherein the carbon-carbon multiple bond is non-polar.
11. The composition of claim 1 wherein the carbon-carbon multiple bond is in a cyclic structure.
12. The composition of claim 1 wherein the macromer comprises a polyvalent radical where each valence is attached to a poly(ethylene glycol)-norbornenyl ester.
13. The composition of claim 1 wherein the macromer comprises a polyvalent radical where each valence is attached to a poly(ethylene glycol)-norbornenyl amide.
14. The composition of claim 12 wherein the poly(ethylene glycol) has a molecular weight in the range from about 5,000 to about 40,000.
15. A biocompatible delivery system comprising a hydrogel formed from the mixture of claim 1 and a biologically active agent wherein the biologically active agent is coated by the hydrogel.
16. The delivery system of claim 15 wherein the biologically active agent is a therapeutic agent, a diagnostic agent, or a combination thereof; a protein or nucleic acid, or combination thereof; an immunologic agent; or a population of cells; or any combination of the foregoing.
17. The delivery system of claim 15 wherein the biocompatible hydrogel is multi-layered, where each layer has independently selected characteristics.