Myelination of congenitally dysmyelinated forebrains using oligodendrocyte progenitor cells
One form of the present invention is directed to a method of remyelinating demyelinated axons by treating the demyelinated axons with oligodendrocyte progenitor cells under conditions which permit remyelination of the axons. Another aspect of the present invention relates to a method of treating a subject having a condition mediated by a loss of myelin or a loss of oligodendrocytes by administering to the subject oligodendrocyte progenitor cells under conditions effective to treat the condition mediated by a loss of myelin or a loss of oligodendrocytes. A further aspect of the present invention relates to an in vitro method of identifying and separating oligodendrocyte progenitor cells from a mixed population containing other mammalian brain or spinal cord cell types.
1. A method of myelinating demyelinated axons of the central nervous system in a subject having a condition mediated by a loss of myelin or a loss of oligodendrocytes, said method comprising:
selecting a subject having a condition mediated by a loss of myelin or a loss of oligodendrocytes and
administering to the selected subject human A2B5 + /PSA-NCAM − oligodendrocyte progenitor cells via intraventricular, intracallosal, or intraparenchymal administration to the brain, brain stem or spinal cord under conditions effective to myelinate demyelinated axons of the central nervous system of the selected subject, wherein the human A2B5 + /PSA-NCAM − oligodendrocyte progenitor cells are separated from a mixed population of human brain or spinal cord cells.
2. The method according to claim 1 , wherein the selected subject has a condition mediated by a loss of oligodendrocytes.
3. The method according to claim 1 , wherein the selected subject has a condition mediated by a loss of myelin.
4. The method according to claim 3 , wherein the condition is an ischemic demyelinating condition.
5. The method according to claim 3 , wherein the condition is an inflammatory demyelinating condition.
6. The method according to claim 5 , wherein the inflammatory demyelinating condition is selected from the group consisting of multiple sclerosis, Schilder's Disease, transverse myelitis, optic neuritis, post-vaccination encephalomyelitis, and post-infectious encephalomyelitis.
7. A method according to claim 3 , wherein the condition is mucopolysaccharidosis.
8. The method according to claim 3 , wherein the condition is perinatal germinal matrix hemorrhage, periventricular leukomalacia, or cerebral palsy.
9. The method according to claim 3 , wherein the condition is a radiation-induced condition.
10. The method according to claim 3 , wherein the condition is an etiology causing sub-cortical leukoencephalopathy, said etiology being HIV/AIDS, head trauma, or multi-infarct states.
11. The method according to claim 1 , wherein the subject is a human.
12. The method according to claim 11 , wherein the subject is a post-natal human.
13. The method according to claim 11 , wherein the subject is an adult human.
14. The method according to claim 1 , wherein the method is carried out in the brain.
15. The method according to claim 1 , wherein the method is carried out in the spinal cord.