Reaction monitoring

1. A method for determining a level of a particular characteristic specific spectrum representing a particular characteristic of a chemical or biochemical reaction, from light which is emanating from at least one reaction vessel of a plurality of reaction vessels in the course of the reaction, the light having a general spectrum comprising a number of different specific spectra including the particular characteristic specific spectrum, at least two of the different specific spectra at least partially overlapping, the method comprising:

conducting the reaction in the at least one reaction vessel, which is arranged so that the light having the general spectrum emanates from the reaction vessel and a received portion of the light is received by a detector comprising a plurality of photodetectors of different types distributed throughout a two-dimensional array of photodetectors, each photodetector of a particular type comprising a photosensor and a particular type of filter that passes light of a particular waveband range, wherein at least two different particular waveband ranges at least partially overlap, and wherein a subset is formed by some or all photodetectors of the same type;

monitoring output from a plurality of subsets of at least two of the different types of photodetectors; and

utilising the outputs from the plurality of subsets to deconvolve the general spectrum of the received portion of the light based on known spectra that could emanate from the at least one reaction vessel during the reaction, so as to differentiate between the different specific spectra and thereby determine a light level of the particular characteristic specific spectrum;

wherein the received portion of the light is monitored repeatedly over a period of time.

2. A method according to claim 1 wherein the particular characteristic specific spectrum is characteristic of a particular reagent or state of a particular reagent within the reaction vessel, or is derived from a single species of fluorophore, present in the reaction.

3. A method according to claim 1 wherein the different types of photodetectors comprise filters of corresponding different types, and the different types of filters comprise at least red, blue and green colour filters, which are arranged in a Bayer pattern.

4. A method according to claim 1 wherein the chemical or biochemical reaction is a polymerase chain reaction (PCR) conducted in the presence of at least one fluorophore chosen from the group consisting of:

fluorophores which intercalate with nucleic acid;

fluorophores which are modified by the PCR process; and

fluorophores which provide for fluorescent energy transfer between them.

5. A method according to claim 4 wherein the fluorophore is a fluorescent label attached to a first oligonucleotide probe which specifically hybridizes to a target nucleic acid sequence of the PCR and wherein the first oligonucleotide probe contains a second fluorophore, which is able to exchange fluorescent energy with the fluorescent label when present together on the probe, wherein a polymerase having 5′-3′ exonuclease activity is utilized in the PCR so as to digest any first probe bound to target nucleic acid during an extension phase of the reaction.

6. A method according to claim 1 wherein the detector is arranged to receive signals from the plurality of reaction vessels simultaneously.

7. A method according to claim 1 wherein the known spectra are determined from calibration reactions emanating light signals having known spectra.

8. A method for determining a level of a particular characteristic specific spectrum representing a particular characteristic of a chemical or biochemical reaction from light which is emitted in the course of the reaction and has a general spectrum comprising a number of different specific spectra including the particular characteristic specific spectrum, at least two of the different specific spectra overlapping, the method comprising:

receiving a received portion of the light from the reaction;

monitoring the received portion of the light using a camera chip having a plurality of integrated colour filters to produce a plurality of different colour channel outputs, wherein different colour filters pass light of different particular waveband ranges, wherein at least two different particular waveband ranges at least partially overlap; and

deconvolving the general spectrum of the received portion of the light using a processing system based on known spectra that could emanate from the reaction vessel during the reaction so as to differentiate between the different specific spectra and thereby determine a light level of the particular characteristic specific spectrum on the basis of the different colour channel outputs of the camera chip;

wherein the received portion of the light is monitored repeatedly over a period of time.

9. A method according to claim 8 wherein the particular characteristic specific spectrum is characteristic of a particular reagent, and wherein the level of more than one different particular characteristic specific spectrum is determined.

10. A method according to claim 8 wherein the known spectra are determined from calibration reactions emanating light signals having known spectra.