Compounds and methods
A molecular tool for use in a method of providing a molecule that is capable of binding a target molecule based on a set of polypeptides. A polypeptide having a sequence selected from SEQ ID NOs 1-32. The polypeptide may be used in a method of screening for a ligand-polypeptide conjugate capable of binding a target molecule for the ligand. A ligand-polypeptide conjugate, useful e.g. in therapy.
1. A molecular tool for use in a method of providing a molecule that is capable of binding a target molecule, said tool comprising a plurality of polypeptides according to any one or several of SEQ ID NOs 1-32, each polypeptide having a ligand capable of binding a target molecule, attached by an amide bond to an amino acid in a position selected from positions 8, 17, 22, and 34 of the polypeptide sequence, and a reporter group for detecting binding to a target molecule, attached by an amide bond to an amino acid in a position selected from positions 15, 10, 25 and 37 of the polypeptide sequence.
2. A molecular tool according to claim 1 , wherein the polypeptides are according to at least 2 different SEQ ID NOs selected from SEQ ID NOs 1-32.
3. A molecular tool according to claim 1 , wherein the ligand of each polypeptide is attached to an amino acid 8, 17, 22, or 34 independently selected from lysine, ornithine and 2,4-diaminobutyric acid and the reporter group of each polypeptide is attached to an amino acid in position 15, 10, 25 or 37 independently selected from lysine, ornithine and 2,4-diaminobutyric acid.
4. A method of screening for a ligand-polypeptide conjugate capable of binding a target molecule, comprising
providing at least one conjugate molecule comprising a polypeptide having a sequence selected from SEQ ID NOs 1-32, said polypeptide having a ligand for the target molecule attached by an amide bond to an amino acid capable of forming an amide bond with the ligand, said amino acid being at a position in the polypeptide sequence selected from positions 8, 17, 22, and 34, and said polypeptide having a reporter group attached by an amide bond to an amino acid capable of forming an amide bond with the reporter group, said amino acid being at a position in the polypeptide sequence selected from positions 15, 10, 25 and 37;
bringing the target molecule in contact with the conjugate molecule; and
detecting a signal from the reporter group.
5. A method according to claim 4 , comprising providing a set of from 2 to 16 conjugate molecules having differing polypeptide sequences selected from SEQ ID NOs 1-32.
6. An isolated polypeptide having a sequence selected from SEQ ID NOs 1-32.
7. An isolated polypeptide according to claim 6 , wherein a ligand capable of binding a target molecule is attached by an amide bond to an amino acid capable of forming an amide bond with the ligand, said amino acid being in a position selected from positions 8, 17, 22, and 34 of the polypeptide sequence.
8. An isolated polypeptide according to claim 7 , wherein the target molecule is a protein or polypeptide.
9. An isolated polypeptide according to claim 6 , wherein a reporter group for detecting binding to a target molecule is attached by an amide bond to an amino acid capable of forming an amide bond with the reporter group, said amino acid being in a position selected from positions 15, 10, 25 and 37 of the polypeptide sequence.
10. An isolated polypeptide that is a dimer of two polypeptides independently selected from the isolated polypeptides according to claim 6 .
11. A plurality of isolated polypeptides comprising at least two different isolated polypeptides according to claim 6 .
12. A plurality of isolated polypeptides according to claim 11 , comprising isolated polypeptides having different SEQ ID NOs selected from SEQ ID NOs 1-32.
13. A molecular tool according to claim 2 , wherein the ligand of each polypeptide is attached to an amino acid 8, 17, 22, or 34 independently selected from lysine, ornithine and 2,4-diaminobutyric acid and the reporter group of each polypeptide is attached to an amino acid in position 15, 10, 25 or 37 independently selected from lysine, ornithine and 2,4-diaminobutyric acid.