Prognosis diagnosis method and prognosis diagnosis kit for sepsis or multiple organ failure
View Patent ↗Disclosed are: a prognosis diagnosis method which can diagnose the prognosis of a patient suffering from sepsis or sepsis-related multiple organ failure in a simple manner and with high accuracy and a prognosis diagnosis kit for use in the prognosis diagnosis method. The prognosis diagnosis method comprises: a first detection step of detecting a liver fatty acid-binding protein contained in urine collected from a subject with a specific antibody; a second detection step of treating the urine with a Redox reagent such as hemin and detecting a liver fatty acid-binding protein contained in the treated urine with the specific antibody; and a comparison step of comparing a detection value obtained in the first step with a detection value obtained in the second step. It is determined that the larger the detection value obtained in the second step compared to that in the first step, the worse the prognosis.
1. A method for determining a prognosis of sepsis or multiple organ failure, comprising:
measuring a first level of liver-type fatty acid-binding protein (L-FABP) contained in a first portion of a urine sample obtained from a subject diagnosed as having sepsis or multiple organ failure with a specific antibody for liver-type fatty acid-binding protein (L-FABP), said first portion not being treated with hemin;
treating a second portion of the urine sample with herein, and measuring a second level of L-FABP contained in the second portion with the specific antibody;
determining an induction ratio as the ratio of the second level of L-FABP to the first level of L-FABP; and
determining the prognosis of sepsis or multiple organ failure in the subject based on the induction ratio, wherein the subject is determined to have a lower likelihood of survival when the induction ratio exceeds a predetermined threshold value and conversely wherein the subject is determined to have a higher likelihood of survival when the induction ratio when the induction ratio is less than or equal to the predetermined threshold value.
2. The method of claim 1 , wherein the treating is conducted under conditions where the concentration of the hemin in the second portion of the urine sample is 0.1 to 100 mM and for a time of about 30 sec to 10 min.
3. The method of claim 1 , further comprising the steps of:
selecting a treatment strategy for the subject, said treatment strategy comprising at least one treatment selected from the group consisting of fluid resuscitation, administration of an antibiotic, blood purification therapy, control of blood glucose level, and administration of corticosteroid or activated protein C; and
administering, said treatment to the subject when the subject is determined to have a lower likelihood of survival based on the induction ratio exceeding the predetermined threshold value.